5 alpha-Androstane-3 alpha 17 beta-diol Glucuronide 3 alpha-Diol G LIA

5 alpha-Androstane-3 alpha 17 beta-diol Glucuronide 3 alpha-Diol G LIA is for Research Use Only

Size: 1×96 wells
Sensitivity: 0.1 ng/mL
Dynamic Range: 0.25–50 ng/mL
Incubation Time: 60 minutes
Sample Type: Serum
Sample Size: 50 μL
Controls Included

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Assay Background

5α-androstane-3α, 17β-diol glucuronide is a C19 steroid and is either abbreviated as 3α-Diol G, 5α diol G or simply, α diol G. It is produced mainly as a metabolite of testosterone and dihydrotestosterone (DHT). It is largely produced in target peripheral tissues such as the skin, especially around hair follicles. The stimulation by large amounts of 3α-Diol G leads to excessive hair formation, notably where hair is not normally present in women. In recent years the interest in the measurement of this steroid has increased among clinical investigators studying women suffering from idiopathic hirsutism. Among the steroids known to be precursors for 3α-Diol G are dehydroepiandrosterone (DHEA), dehydroepiandrosterone sulfate (DHEAS), dihydrotestosterone (DHT), androstenedione and testosterone. Only 3α-Diol G has been shown to increase with hirsutism and decrease with treatment. This correlation has also been demonstrated in patients with polycystic ovarian syndrome (PCO). 3 α-Diol G determinations have therefore proved to be a useful indicator in a variety of ways including monitoring the progress of treatment of idiopathic hirsutism and women with PCO. Furthermore, diabetic patients (both men and women) under cyclosporine A therapy have shown increased 3 α-Diol G levels, a side effect resulting in the appearance of hair in previously hairless areas.

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SPECIMEN COLLECTION AND STORAGE
Approximately 0.2 mL of serum is required per duplicate determination. Collect 4–5 mL of blood into an appropriately labelled tube and allow it to clot. Centrifuge and carefully remove the serum layer. Store at 4°C for up to 24 hours or at -10°C or lower if the analyses are to be done at a later date. Consider all human specimens as possible biohazardous materials and take appropriate precautions when handling.

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