Hydroxypyridinium Crosslinks HPLC Column

The Hydroxypyridinium Crosslinks HPLC Column is For Research Use Only

Each column is can be utilized for approximately 500-1000 samples depending on column care and sample type.

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Assay Background

Pyridinium and deoxypyridinium are built as intramolecular crosslinkers during the maturation of collagen. During bone resorption collagen is degraded and the pyridinium-crosslinks are excreted. They are not re-utilized by the body and excreted in the urine. The crosslinks are not released during the de novo synthesis of collagen. They are specific for resorptive processes. Neither PYD nor DPD are taken up by nutrition. Therefore the determination of the crosslinks is independent from the diet. PYD is found in cartilage, ligaments and bone, whereas DPD is rather specific for bone.

The crosslink excretion shows a strong circadian rhythm with a maximum in the early morning hours. Reliable data can be obtained with a 24h or a creatinine corrected morning urine. An elevated crosslink excretion is shown in osteoporotic women. Crosslink levels decrease during treatment with estrogen (Seibel et al. 1994). Therefore they are suited for therapy monitoring (Ganero et al. 1994). The measurement of pyridinium crosslinks is an independent predictor for future bone fractures (Ganero et al. 1995). Increased crosslink concentrations are also described in patients with Paget’s disease, primary hyperparathyroidism and bone metastasis.

Hydroxypyridinium Crosslinks HPLC Assay kit application for the pyridinium crosslinks makes it possible to determine pyridinium and deoxypyridinium in an easy, fast and precise method. The Hydroxypyridinium Crosslinks HPLC Assay kit includes all reagents in a ready to use form for preparation and separation of the samples with exception of the columns (IC3201rp) and the controls (IC3201ko). Both can be supplied separately by Eagle Biosciences. Beside the complete test kits it is possible to order all components separately. Please request our single component price list.

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