iLite FGF-21 Assay Ready Cells
iLite FGF-21 Assay Ready Cells Developed and Manufactured by Svar Life Science
The Eagle Biosciences iLite® FGF-21 Assay Ready Cells are intended for :
- Testing FGF-21 functional activity
- Testing NAb against FGF-21
Receipt and Storage: Upon receipt confirm that adequate dry-ice is present and the cells are frozen. Immediately transfer to -80°C storage. Cells should be stored at -80°C (do not store at any other temperature) and are stable as supplied until the expiry date shown. Cells should be used within 30 min of thawing and should be diluted immediately after thawing.
For Research Use Only
Key benefits of iLite® Assays
- Highly specific reporter gene cell lines
- Very sensitive cell line responses (>10 fold inductions)
- Assay Ready Cells – ready-to-use from the freezer, without culturing of cells
- Assays within a workday (typically 4-7 hour assays)
- Normalization gene, which eliminates unwanted matrix effects
Background
Human fibroblast growth factor 21 (FGF21) is a member of a family of the atypical fibroblast growth factors that include FGF19 and FGF23 in man. FGF21 lacks the heparin-binding domain of conventional FGFs and can consequently diffuse throughout the body and function as a hormone. FGF21 stimulates glucose uptake in adipocytes and the effects on glucose uptake is additive with insulin (1). The iLite® FGF21 ARCs are a genetically engineered reporter gene cell line responsive to FGF21 by specific and proportional expression of Firefly Luciferase. The receptor chain FGFR1c is overexpressed on the surface together with the co-factor β-Klotho which has been genetically optimized to enhance sensitivity. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.
Products Related to FGF-21 Assay Ready Cells
Total FGF-21 ELISA Assay
cTerminal FGF21 ELISA Kit
N-Terminal FGF21 ELISA Kit
Related News
New Product Introduction
EagleBio的iLite®分析就绪细胞iLite® 技术基于报告基因检测格式，经过修改和适用于整个药物开发周期中的应用以及生物药物的监测。这些细胞系可以开发用于任何生物制药靶标，并且可以使用相同的细胞系轻松设置药物效力（即药物活性）的测定方法。检验就绪的细胞经过基因工程处理，可与报告基因检验技术一起使用，以检测药物效力和NAb。
