Recombinant Mouse His6-USP18 Protein, CF Summary

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: USP18

Ubiquitin-like carboxyl-terminal hydrolase 18 (USP18) is a specialized cysteine protease with a predicted molecular weight of 42 kDa. USP18 from Mus musculus is a member of the peptidase C19 family and shares 70% amino acid identity with its human ortholog. USP18 plays and important role in antiviral responses and interferon-mediated signaling pathways. Mice lacking the gene (USP18^-/-) are hypersensitive to interferon, have shortened lifespans and pathologic abnormalaties of the brain. While it was initially speculated that the cause of these phenotypesstems from the accumulaiton of ISG15-conjugated intracellular proteins (a target for USP18 catalytic function), it was later demonstrated that USP18 has multiple roles, at least one of which is independent of its ISGylase activity.

Related Research Areas

• Deubiquitinating Enzymes (DUBs)
• IL-10 and Interferon Family Regulators
• Search Ubiquitin-related Enzymes
• Ubiquitin-like Modifiers (UBLs) and Regulators