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Recombinant Tri-ubiquitin/Ub3 K11/K48 branched Protein, CF Summary
Ub-AQUA analysis:
K11 + K48: 99%
All other linkages ≤ 0.5%
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
UC-1148
| Formulation | 0.5 mg/ml (20μM) in 10 mM HEPES pH 7.5 |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Tri-Ubiquitin
Linkage-specific polyubiquitin chains may be used as a substrate for in vitro reactions with deubiquitinating enzymes ("DUB"s") that cleave the peptide of isopeptide linkage between adjacent ubiquitin molecules. Polyubiquitin chains can also be used to investigate mechanisms of binding and recognition between the chains and other proteins that contain Ubiquitin-Associated domains (UBA"s), Ubiquitin-Interacting Motifs (UIM"s), ZnF"s, and/or other ubiquitin-sensing elements.
K11/K48-branched polyubiquitin chains facilitate rapid substrate delivery to the proteasome, which prevents accumulation of aggregation-prone nascent proteins. Furthermore, enzymes and effectors of K11/K48-linked chains are encoded by essential genes that are often mutatedin neurodegenerative diseases, providing proof that heterotypic ubiquitin chains play important roles in cellular signaling pathways.
The correctness of linkage and purity of each production lot of this product is assessed using the Absolute Quantitation of Ubiquitin Method (Ub-AQUA), an LCMS-based technique that provides extremely accurate information of the composition of polyubiquitin samples. The product is also tested in a Western Blot application described by Yau et al.
- Yau, R.G. et al. (2017) Cell: doi.org/10.1016/j.cell.2017.09.040
FAQs
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