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蚂蚁淘在线 / 品牌中心 / Boston Biochem / 波士顿生物化学/重组人His6-NEDD8蛋白，CF/UL-813-500

波士顿生物化学/重组人His6-NEDD8蛋白，CF/UL-813-500

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￥3180.00
货号：UL-813-500
浏览量：67
品牌：Boston Biochem
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SummaryProduct DatasheetsCarrier FreeReconstitution CalculatorBackgroundRelated Research Areas

Recombinant Human His6-NEDD8 Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-NEDD8 can be conjugated to substrate proteins via the subsequent actions of a NEDD8-activating (E1) enzyme, a NEDD8-conjugating (E2) enzyme, and a NEDD8 ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-NEDD8 concentration of 5-20 μM.
Source
E. coli-derived human NEDD8 proteinContains a 6-His tagAccession # Q15843
Accession #
Q15843
Predicted Molecular Mass
9.5 kDa

Product Datasheets

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

UL-813

FormulationSupplied as a solution in Tris, NaCl and Glycerol.
ShippingThe product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NEDD8

Neural Precursor Cell Expressed Developmentally Downregulated Gene 8 (NEDD8), also known as Related to Ubiquitin 1 (Rub1), is a 6-8 kDa member of the Ubiquitin family of proteins. Human pro-NEDD8 is 81 amino acids (aa) in length. Pro-NEDD8 contains one Ubiquitin-like domain (aa 1-65) and a 4-5 aa C-terminal propeptide (1,2). Cleavage of the propeptide exposes a C-terminal glycine residue that is used to generate a glycine-lysine intermolecular bond. Mature human NEDD8 shows 100% aa identity to NEDD8 from mouse, rat, and canine. Human NEDD8 is activated by a distinct NEDD8-activating (E1) enzyme, a heterodimeric complex composed of APPBP1 and UBA3 subunits (3). Activated NEDD8 is subsequently transferred to the UBE2M/Ubc12 or UBE2F NEDD8-conjugating (E2) enzymes (4). Through a process termed neddylation, the ROC1/Rbx1 RING Finger E3 ligase transfers NEDD8 to specific substrates (5). For example, transfer of NEDD8 to the Cullin scaffold activates members of the Cullin-RING Ligase (CRL) family of Ubiquitin ligases (E3) (6-8). CRL-induced ubiquitination is known to regulate proteins that are important for cell cycle progression and cell survival (9,10). Physiologically, NEDD8 plays a critical regulatory role in cell proliferation and dysregulation of the NEDD8 pathway has been associated with several cancer pathologies (11,12).

References
  1. Kumar, S. et al. (1993) Biochem. Biophys. Res. Commun. 195:393.
  2. Kamitani, T. et al. (1997) J. Biol. Chem. 272:28557.
  3. Huang, D.T. & B.A. Schulman (2005) Methods Enzymol. 398:9.
  4. Gong, L. & E.T. Yeh (1999) J. Biol. Chem. 274:12036.
  5. Ohki, Y. et al. (2009) Biochem. Biophys. Res. Commun. 381:443.
  6. Petroski, M.D. & R.T. Deshares (2005) Nat. Rev. Mol. Cell Biol. 6:9.
  7. Hjerpe, R. et al. (2012) Biochem. J. 441:927.
  8. Calabrese, M.F. et al. (2011) Nat. Struct. Mol. Biol. 18:947.
  9. Tateishi, K. et al. (2001) J. Cell. Biol. 155:571.
  10. Ohh, M. et al. (2002) EMBO Rep. 3:177.
  11. Luo, Z. et al. (2012) Cancer Res. 72:3360.
  12. Soucy, T.A. et al. (2010) Genes Cancer 1:708.
Long Name
Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8
Entrez Gene IDs
4738 (Human); 18002 (Mouse); 25490 (Rat)
Alternate Names
FLJ43224; MGC104393; MGC125896; MGC125897; NEDD8; NEDD-8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; neural precursor cell expressed, developmentally down-regulated 8; Rub1; Ubiquitin-like protein Nedd8

FAQs

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Recombinant Enzymes

Recombinant Human His6-UBE2F Protein, CF

E2-679
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Recombinant Human UBE2M/Ubc12 Protein, CF

E2-656
4Citations
EnzAct

Recombinant Human NEDD8 E1 (APPBP1/UBA3) Protein, CF

E-313
5Citations
EnzAct

Recombinant Human His6-NEDP1/SENP8 Protein, CF

E-800
1Review
EnzAct

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Boston Biochem2011年，波士顿生物化学被TECHNE Corporation的 R＆D Systems收购，R＆D Systems是细胞生物学试剂的领先供应商，其拥有20多年的经验和独特的产品制造模式，可确保严格的质量控制以及产品中最高的一致性和性能。  凭借R＆D System在销售，分销和物流方面的专业知识，Boston Biochem现在专注于新产品开发。在蛋白质纯化，表征，修饰和分析开发方面的广泛专业知识以及与UPP研究社区的紧密联系使我们能够继续提供最全面的产品范围。我们的收购也使我们有机会共同开发R＆D Systems的互补抗体，ELISA和试剂盒以及Tocris Bioscience的互补抑制剂和底物。波士顿生物化学的所有专用工具将继续在内部进行开发，生产和表征，并致力于提供可负担性和卓越的产品。  2014年，Techne成为Bio-Techne，并与Boston Biochem和R＆D Systems和Tocris的其他核心品牌一起为研究人员提供了广泛的产品和服务。随着我们成为波士顿生物化学品牌-Bio-Techne品牌，您可以期望我们提供与您相同的泛素专业知识和质量。