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蚂蚁淘在线 / 品牌中心 / Boston Biochem / 波士顿生物化学/重组人GST-UbcH2/UBE2H蛋白，CF/E2-605-100

波士顿生物化学/重组人GST-UbcH2/UBE2H蛋白，CF/E2-605-100

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￥4780.00
货号：E2-605-100
浏览量：127
品牌：Boston Biochem
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SummaryProduct DatasheetsCarrier FreeReconstitution CalculatorBackgroundRelated Research Areas

Recombinant Human GST-UbcH2/UBE2H Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human GST-UbcH2/UBE2H is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-UbcH2/UBE2H concentration of 0.1-1 μM.
Source
E. coli-derived human UbcH2/UBE2H proteinContains an N-terminal GST (glutathione S-transferase) tag
Accession #
P62256
Predicted Molecular Mass
46 kDa

Product Datasheets

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-605

FormulationX mg/ml (XμM) in 50 mM HEPES, pH 8.0, 100 mM NaCl, and 10% Glycerol (v/v)
ShippingThe product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UbcH2/UBE2H

Ubiquitin-conjugating Enzyme H2 (UbcH2), also known as Ubiquitin-conjugating Enzyme E2H (UBE2H), is a member of the Ubiquitin-conjugating (E2) enzyme family (1). UbcH2/UBE2H has a predicted molecular weight of 20.5 kDa. The human protein shares 100% amino acid sequence identity with the mouse and rat orthologs. Human UbcH2/UBE2H is highly expressed in skeletal and cardiac muscle, shows significant expression levels in kidney and placenta, and is expressed at low levels in liver, brain, lung, and spleen (2). TNF-alpha induces UbcH2/UBE2H expression via NF-kappa B, and SCL/Tal1 upregulates the expression of UbcH2/UBE2H during erythroid differentiation (2,3). Pathologically, a single nucleotide polymorphism within UbcH2/UBE2H is associated with autism (4). Furthermore, UbcH2/UBE2H has been reported as a putative oncogene for liver tumorigenesis (5). This protein has an N-terminal GST-tag. (Accession # NP_003335)

References
  1. Kaiser, P. et al. (1994) J. Biol. Chem. 269:8797.
  2. Li, Y.P. et al. (2003) FASEB J. 17:1048.
  3. Lausen, J. et al. (2010) J. Biol. Chem. 285:5338.
  4. Vourc"h, P. et al. (2003) Psychiatr. Genet. 13:221.
  5. Keng, V.W. et al. (2009) Nat. Biotechnol. 27:264.
Entrez Gene IDs
7328 (Human); 22214 (Mouse)
Alternate Names
E2-14K; E2-20K; EC 6.3.2.19; UBC8; UBC8E2-20K; UBCH; UbcH2; UBE2H; Ubiquitin carrier protein H; ubiquitin-conjugating enzyme E2 H; Ubiquitin-conjugating enzyme E2-20K; ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8); ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast); Ubiquitin-protein ligase H

FAQs

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Boston Biochem2011年，波士顿生物化学被TECHNE Corporation的 R＆D Systems收购，R＆D Systems是细胞生物学试剂的领先供应商，其拥有20多年的经验和独特的产品制造模式，可确保严格的质量控制以及产品中最高的一致性和性能。  凭借R＆D System在销售，分销和物流方面的专业知识，Boston Biochem现在专注于新产品开发。在蛋白质纯化，表征，修饰和分析开发方面的广泛专业知识以及与UPP研究社区的紧密联系使我们能够继续提供最全面的产品范围。我们的收购也使我们有机会共同开发R＆D Systems的互补抗体，ELISA和试剂盒以及Tocris Bioscience的互补抑制剂和底物。波士顿生物化学的所有专用工具将继续在内部进行开发，生产和表征，并致力于提供可负担性和卓越的产品。  2014年，Techne成为Bio-Techne，并与Boston Biochem和R＆D Systems和Tocris的其他核心品牌一起为研究人员提供了广泛的产品和服务。随着我们成为波士顿生物化学品牌-Bio-Techne品牌，您可以期望我们提供与您相同的泛素专业知识和质量。