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蚂蚁淘在线 / 品牌中心 / Boston Biochem / Boston Biochem/Recombinant Human GST-UbcH5c/UBE2D3 Protein, CF/E2-625-100

Boston Biochem/Recombinant Human GST-UbcH5c/UBE2D3 Protein, CF/E2-625-100

价格
￥4780.00
货号：E2-625-100
浏览量：127
品牌：Boston Biochem
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SummaryProduct DatasheetsCarrier FreeReconstitution CalculatorBackgroundRelated Research Areas

Recombinant Human GST-UbcH5c/UBE2D3 Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human GST-UbcH5c/UBE2D3 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-UbcH5c/UBE2D3 concentration of 0.1-1 μM.
Source
E. coli-derived human UbcH5c/UBE2D3 proteinContains an N-terminal GST (glutathione S-transferase) tag
Accession #
P61077
Predicted Molecular Mass
44 kDa

Product Datasheets

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-625

FormulationX mg/ml (X μM) in 50 mM HEPES pH 7.0, 200 mM NaCl, 10% Glycerol, 1 mM TCEP
ShippingThe product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UbcH5c/UBE2D3

Ubiquitin-conjugating Enzyme H5c (UbcH5c), also known as Ubiquitin-conjugating Enzyme E2D 3 (UBE2D3), is a member of the yeast Ubc4/5 family of Ubiquitin-conjugating (E2) enzymes. Human UbcH5c/UBE2D3 has a predicted molecular weight of 17 kDa and shares 88% and 89% amino acid sequence identity with the related family members, UbcH5a and UbcH5b, respectively. In combination with Ubiquitin ligases (E3s) such as CHIP, UbcH5c/UBE2D3 mediates the ubiquitination and subsequent degradation of several regulatory proteins (1). For instance, UbcH5c/UBE2D3 is involved in the poly-ubiquitination and proteasome-mediated degradation of the Nuclear Factor kappaB (NF-kappaB) inhibitor, IkappaB-alpha, and is implicated in NF-kappaB-dependent inflammation (2-4). UbcH5c/UBE2D3 also mediates the ubiquitination of Histone H2A and PCNA, suggesting that it functions during transcriptional regulation, DNA replication, and DNA damage responses (5-7).

References
  1. Page, R.C. et al. (2012) Biochemistry 51:4175.
  2. Gonen, H. et al. (1999) J. Biol. Chem. 274:14823.
  3. Shembade, N. et al. (2010) Science 327:1135.
  4. Xia, Z.P. et al. (2009) Nature 461:114.
  5. Zhang, S. et al. (2008) Cell Cycle 7:3399.
  6. Bentley, M.L. et al. (2011) EMBO J. 30:3285.
  7. Polanowska, J. et al. (2006) EMBO J. 25:2178. 
Entrez Gene IDs
7323 (Human); 66105 (Mouse); 81920 (Rat)
Alternate Names
E2(17)KB 3; E2(17)KB3; EC 6.3.2.19; MGC43926; MGC5416; UBC4/5; UBC5C; UbcH5c; UBE2D3; Ubiquitin carrier protein D3; ubiquitin-conjugating enzyme E2 D3; Ubiquitin-conjugating enzyme E2(17)KB 3; Ubiquitin-conjugating enzyme E2-17 kDa 3; ubiquitin-conjugating enzyme E2D 3 (homologous to yeast UBC4/5); ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase D3

Citations for Recombinant Human GST-UbcH5c/UBE2D3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.

2Citations: Showing 1 - 2Filter your results:

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  1. Sec16A, a key protein in COPII vesicle formation, regulates the stability and localization of the novel ubiquitin ligase RNF183Authors: Y Wu, XP Guo, S Kanemoto, Y Maeoka, A Saito, R Asada, K Matsuhisa, Y Ohtake, K Imaizumi, M KanekoPLoS ONE, 2018;13(1):e0190407.Applications: Bioassay
  2. Genome-wide identification and gene expression profiling of ubiquitin ligases for endoplasmic reticulum protein degradationSci Rep, 2016;6(0):30955.Species: HumanSample Types: ProteinApplications: Enzyme Assay

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Reviews for Recombinant Human GST-UbcH5c/UBE2D3 Protein, CF

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Recombinant Human GST-UbcH5c/UBE2D3 Protein, CF
By Anonymouson 09/10/2018
Application: Binding assay/Protein-protein interaction
Recombinant Human GST-UbcH5c/UBE2D3 Protein, CF
By Anonymouson 09/10/2018
Application: Enzymatic activity in vitro
Boston Biochem2011年，波士顿生物化学被TECHNE Corporation的 R＆D Systems收购，R＆D Systems是细胞生物学试剂的领先供应商，其拥有20多年的经验和独特的产品制造模式，可确保严格的质量控制以及产品中最高的一致性和性能。  凭借R＆D System在销售，分销和物流方面的专业知识，Boston Biochem现在专注于新产品开发。在蛋白质纯化，表征，修饰和分析开发方面的广泛专业知识以及与UPP研究社区的紧密联系使我们能够继续提供最全面的产品范围。我们的收购也使我们有机会共同开发R＆D Systems的互补抗体，ELISA和试剂盒以及Tocris Bioscience的互补抑制剂和底物。波士顿生物化学的所有专用工具将继续在内部进行开发，生产和表征，并致力于提供可负担性和卓越的产品。  2014年，Techne成为Bio-Techne，并与Boston Biochem和R＆D Systems和Tocris的其他核心品牌一起为研究人员提供了广泛的产品和服务。随着我们成为波士顿生物化学品牌-Bio-Techne品牌，您可以期望我们提供与您相同的泛素专业知识和质量。