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Ac-Ala-Asn-Trp-AMC (Ac-ANW-AMC) Summary
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Citations for Ac-Ala-Asn-Trp-AMC (Ac-ANW-AMC)
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
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- H727 cells are inherently resistant to the proteasome inhibitor carfilzomib, yet require proteasome activity for cell survival and growthAuthors: MJ Lee, Z Miller, JE Park, D Bhattarai, W Lee, KB KimSci Rep, 2019;9(1):4089.2019
- Immunoproteasome functions explained by divergence in cleavage specificity and regulationAuthors: MB Winter, F La Greca, S Arastu-Kap, F Caiazza, P Cimermanci, TJ Buchholz, JL Anderl, M Ravalin, MF Bohn, A Sali, AJ O"Donoghue, CS CraikElife, 2017;6(0):.2017
- Brief treatment with a highly selective immunoproteasome inhibitor promotes long-term cardiac allograft acceptance in miceProc. Natl. Acad. Sci. U.S.A, 2016;113(52):E8425-E8432.2016
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S-320 hydrolysis confirmation by LCMS-SRMS-320: 1 uMi20S: 15 nMPA28a: 150 nMReaction: 37 °C for 5 h1:S-320 Ac-ANW-AMC (589.15>414.15)2:Free AMC (176.25>120.00)3:S-320 Ac-ANW-COOH (432.20>319.15)
Ordinate: fluorescent count, Em 345/Ex 445Em band path: 9 nmEx band path: 20 nmHorizontal: time [min]Temp: 37 °CS-320: 5, 25, 50, and 100 uMi20S: 100 ng per assay (100 uL)The linearity of the kinetics was confirmed, and the fluorescence intensity increased in a concentration-dependent manner. But it is considered that the saturated level has not been reached using this condition. Probably it could be a low amount of i20S.
