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Recombinant Human MYSM1 Protein, CF Summary
Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant humanMYSM1 concentration of 20-100 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-598
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 2 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: MYSM1
Histone H2A deubiquitinase MYSM1 is a specialized metalloprotease with a predicted molecular weight of 95 kDa. MYSM1 is a member of the peptidase M67A family and the human protein shares 79% amino acid sequence identity with its mouse ortholog. MYSM1 has been reported to function within a large chromatin remodeling complex, containing itself, PCAF, RBM10, TRIP5, and possibly other proteins. Biologically speaking, MYSM1 plays roles in hematopoiesis and lymphocyte differentiation, stem cell maintenance, and innate immunity.
- Belle J.I., et al. (2016) Cell Death Differ. 2016 doi: 10.1038/cdd.2015.140.
- Le Guen T., et al. (2015) J Allergy Clin. Immunol. 136: 1619
- Panda S, Nilsson J.A., Gekara N.O. (2015) Immunity 43: 647
- Zhu P., et al. (2007) Mol. Cell 27: 609
FAQs
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