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蚂蚁淘在线 / 品牌中心 / Boston Biochem / 波士顿生物化学/重组人FLAG（DYKDDDDK）-泛素蛋白，CF/U-120-01M

波士顿生物化学/重组人FLAG（DYKDDDDK）-泛素蛋白，CF/U-120-01M

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￥1300.00
货号：U-120-01M
浏览量：73
品牌：Boston Biochem
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Citations (13)
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SummaryProduct DatasheetsCarrier FreeReconstitution CalculatorBackgroundRelated Research Areas

Recombinant Human FLAG (DYKDDDDK)-Ubiquitin Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human FLAG-Ubiquitin can be conjugated to substrate proteins via the subsequent actions of a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human FLAG-Ubiquitin concentration of 0.5-1 mM.
Source
E. coli-derived human Ubiquitin proteinContains an N-terminal FLAG (DYKDDDDK) tag
Accession #
P0CG47
Predicted Molecular Mass
9.8 kDa

Product Datasheets

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

U-120

FormulationLyophilized from a solution in deionized water.
ReconstitutionReconstitute at 10 mg/mL in an aqueous solution.
ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ubiquitin

Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1) (2,3). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting (4-7).

This fully functional N-terminal FLAG-tagged Ubiquitin protein allows for the convenient detection or affinity purification of ubiquitinated proteins in vitro. The epitope tag is a hydrophilic octapeptide (DYKDDDDK) derived from the sequence of the bacteriophage 7 gene-10 product. This tag is specifically recognized by Anti-FLAG antibodies M1, M2 or M5.

References
  1. Sharp, P.M. & W.-H. Li. (1987) Trends Ecol. Evol. 2:328.
  2. Ciechanover, A. et al. (1980 ) Proc. Natl. Acad. Sci. USA 77:1365.
  3. Hershko, A. et al. (1980) Proc. Natl. Acad. Sci. USA 77:1783.
  4. Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
  5. Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
  6. Wei, W. et al. (2004) Nature 428:194.
  7. Wertz, I.E. et al. (2004) Nature 430:694.
Entrez Gene IDs
7314 (Human); 298693 (Rat)
Alternate Names
RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin

Citations for Recombinant Human FLAG (DYKDDDDK)-Ubiquitin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.

13Citations: Showing 1 - 10Filter your results:

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  1. Ubiquitination of MAP1LC3B by pVHL is associated with autophagy and cell death in renal cell carcinomaAuthors: HM Kang, KH Noh, TK Chang, D Park, HS Cho, JH Lim, CR JungCell Death Dis, 2019;10(4):279.Applications: Bioassay
  2. Ring finger protein 145 (RNF145) is a ubiquitin ligase for sterol-induced degradation of HMG-CoA reductaseAuthors: LY Jiang, W Jiang, N Tian, YN Xiong, J Liu, J Wei, KY Wu, J Luo, XJ Shi, BL SongJ. Biol. Chem., 2018;0(0):.Applications: Bioassay
  3. Proteasome-associated HECT-type ubiquitin ligase activity is required for plant immunityAuthors: JJ Furniss, H Grey, Z Wang, M Nomoto, L Jackson, Y Tada, SH SpoelPLoS Pathog., 2018;14(11):e1007447.Species: HumanSample Types: ProteinApplications: Bioassay
  4. The Ubiquitin Ligase CHIP Integrates Proteostasis and Aging by Regulation of Insulin Receptor TurnoverAuthors: R Tawo, W Pokrzywa, É Kevei, ME Akyuz, V Balaji, S Adrian, J Höhfeld, T HoppeCell, 2017;169(3):470-482.e13.Applications: Bioassay
  5. Reciprocal Regulation between 53BP1 and the Anaphase-Promoting Complex/Cyclosome Is Required for Genomic Stability during Mitotic StressAuthors: TJ Kucharski, PE Minshall, M Moustafa-K, AS Turnell, JG TeodoroCell Rep, 2017;18(8):1982-1995.
  6. Ufd2p synthesizes branched ubiquitin chains to promote the degradation of substrates modified with atypical chainsAuthors: C Liu, W Liu, Y Ye, W LiNat Commun, 2017;8(0):14274.Species: YeastSample Types: Cell LysatesApplications: Bioassay
  7. COP1 mediates dark-specific degradation of microtubule-associated protein WDL3 in regulating Arabidopsis hypocotyl elongationAuthors: N Lian, X Liu, X Wang, Y Zhou, H Li, J Li, T MaoProc. Natl. Acad. Sci. U.S.A., 2017;114(46):12321-12326.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
  8. Ubiquitin- and ATP-dependent unfoldase activity of P97/VCP�NPLOC4�UFD1L is enhanced by a mutation that causes multisystem proteinopathyAuthors: EE Blythe, KC Olson, V Chau, RJ DeshaiesProc. Natl. Acad. Sci. U.S.A., 2017;0(0):.Applications: Bioassay
  9. Hemi-methylated DNA regulates DNA methylation inheritance through allosteric activation of H3 ubiquitylation by UHRF1Elife, 2016;5(0):.Species: HumanSample Types: ProteinApplications: Ubiquitination
  10. Dimeric Ube2g2 simultaneously engages donor and acceptor ubiquitins to form Lys48-linked ubiquitin chains.Authors: Liu, Weixiao, Shang, Yonglian, Zeng, Yan, Liu, Chao, Li, Yanchang, Zhai, Linhui, Wang, Pan, Lou, Jizhong, Xu, Ping, Ye, Yihong, Li, WeiEMBO J, 2014;33(1):46-61.Species: N/ASample Types: Recombinant ProteinApplications: Bioassay
  11. The Rad6/18 ubiquitin complex interacts with the Epstein-Barr virus deubiquitinating enzyme, BPLF1, and contributes to virus infectivity.Authors: Kumar, Ravindra, Whitehurst, Christop, Pagano, Joseph SJ Virol, 2014;88(11):6411-22.Species: N/ASample Types: ProteinApplications: Bioassay
  12. Cytosolic quality control of mislocalized proteins requires RNF126 recruitment toBag6.Authors: Rodrigo-Brenni M, Gutierrez E, Hegde RMol Cell, 2014;55(2):227-37.Applications: Bioassay
  13. An unusual two-step control of CPEB destruction by Pin1.Authors: Nechama, Morris, Lin, Chien-Li, Richter, Joel DMol Cell Biol, 2013;33(1):48-58.Species: XenopusSample Types: In VivoApplications: In Vivo

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Reconstitution Buffers

Reconstitution Buffer 1 (PBS)

RB01
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Boston Biochem2011年，波士顿生物化学被TECHNE Corporation的 R＆D Systems收购，R＆D Systems是细胞生物学试剂的领先供应商，其拥有20多年的经验和独特的产品制造模式，可确保严格的质量控制以及产品中最高的一致性和性能。  凭借R＆D System在销售，分销和物流方面的专业知识，Boston Biochem现在专注于新产品开发。在蛋白质纯化，表征，修饰和分析开发方面的广泛专业知识以及与UPP研究社区的紧密联系使我们能够继续提供最全面的产品范围。我们的收购也使我们有机会共同开发R＆D Systems的互补抗体，ELISA和试剂盒以及Tocris Bioscience的互补抑制剂和底物。波士顿生物化学的所有专用工具将继续在内部进行开发，生产和表征，并致力于提供可负担性和卓越的产品。  2014年，Techne成为Bio-Techne，并与Boston Biochem和R＆D Systems和Tocris的其他核心品牌一起为研究人员提供了广泛的产品和服务。随着我们成为波士顿生物化学品牌-Bio-Techne品牌，您可以期望我们提供与您相同的泛素专业知识和质量。