- 清关代理品牌怎么样清关代理品牌介绍、联系方式品牌网
- 活动前后,买家要求退差价合理吗?不退算违规吗?_开淘网
- Bostonbiochem(boston biochem) 代理蚂蚁淘原厂直采
- Boston biochem代理Boston biochem代理Boston biochem北京代理...
- 海南航空官网_机票查询_航班查询_特价机票_网上值机
- Boston Biochem全国代理_Boston Biochem北京百奥创新科技有限...
- 虎扑体育网 hupu.com
- 利用DIS系统对伽利略理想实验进行数据呈现
- Bostonbiochem(boston biochem) 代理蚂蚁淘原厂直采
- BostonBiochem最全面泛素研究工具库_公司新闻
- Boston BioProducts代理品牌：Boston BioProducts代理美 ...
- chondrex中国代理商2020 |产品目录|代理商|有限...
Recombinant SARS-CoV-2 3CL Protease, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-720
| Formulation | 1.7 mg/ml (50 μM) in 50 mM HEPES, pH 7.5, 150 mM NaCl, 1 mM DTT, 1 mM EDTA |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Data Images
View LargerGC376 achieves 50% inhibition of 30 nM SARS-CoV-2 3CL Protease (E-720) at a concentation of 54-71 nM.
View LargerSDS-PAGE of Recombinant SARS-CoV-2 3CL Protease. 1 μg and 2 μg of Recombinant SARS-CoV-2 3CL Protease (Catalog # E-720) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 34 kDa.
View LargerLC-MS analysis of SARS-CoV-2 3CL Protease. SARS-CoV-2 3CL Protease (Catalog # E-720) was analyzed by a Thermo Q Exactive HF instrument then deconvoluted using Thermo BioPharma Finder software. The expected monoisotopic mass is 33774.478 Da.
Reconstitution Calculator
Background: 3CL Protease
The 3CL protease (aka 3CLpro, Mpro or "Main" Protease) from the human SARS-CoV-2 coronavirus (Severe Acute Respiratory Syndrome coronavirus 2) is a C30-type cysteine protease located within the non-structural proteins 3 (NS3) section of the viral polypeptide.3CLproactivity is required to process the viral polyprotein into functional, mature subunits, and there are 11 or more sites of cleavage, many containing the sequence LQ[S/A/G]; the protease cleaves c-terminal to the glutamine amino acid. Along with the CoV-2 Papain-Like Protease (catalog number E-611),3CLpropresents an attractive target for therapeutic intervention for COVID-19. Because no human proteases with a similar cleavage specificity are known, inhibitors of3CLproare unlikely to cause mechanism-based toxicity. This recombinant protein is untagged.
- Chen Y.W., et al. (2020) F1000Research doi: 10.12688/f1000research.22457.2
- Pillaiyar T., et al. (2016) J. Med. Chem. 59: 6595
- Zhang L., et al. (2020) Science doi: 10.1126/science.abb3405
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant SARS-CoV-2 3CL Protease, CF
There are currently no reviews for this product. Be the first toreview Recombinant SARS-CoV-2 3CL Protease, CF and earn rewards!
Have you used Recombinant SARS-CoV-2 3CL Protease, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
