Boston Biochem/Recombinant Human Linear Tetra-Ub Non-hydrolyzable, CF/UCN-710-100

价格
¥2580.00
货号:UCN-710-100
浏览量:127
品牌:Boston Biochem
服务
全国联保
正品保证
正规发票
签订合同
商品描述
Product Details
FAQs
Supplemental Products
Reviews
SummaryProduct DatasheetsCarrier FreeReconstitution CalculatorBackgroundRelated Research Areas

Recombinant Human Linear Tetra-Ub Non-hydrolyzable, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Ubiquitin chains vary in length, linkage, and function. -linked Linear Non-hydrolyzable Tetra-Ubiquitin Chains (Ub4) may be useful for investigating Ubiquitin-binding proteins and exploring the role of unanchored Ubiquitin chains in signaling pathways. Reaction conditions will need to be optimized for each specific application. IMPORTANT: Heating this product in SDS-PAGE buffer or terminating reactions containing this product with heated SDS-PAGE buffer could lead to unexpected, high apparent molecular weight banding or smearing on gels that is not representative of product purity. For optimal results, we recommend incubation in SDS-PAGE buffer + DTT at <40 °c="" for="" 20="" minutes="" prior="" to="" gel="">
Source
E. coli-derived human Tetra-Ubiquitin protein

Each Ubiquitin contains a Pro substitution at position 73.

Accession #
P0CG47
Predicted Molecular Mass
34 kDa

Product Datasheets

Product Datasheet
COA

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

UCN-710

FormulationLyophilized from a solution in deionized water.
ReconstitutionReconstitute at 2 mg/ml in aqueous buffer.
ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Tetra-Ubiquitin

With a predicted molecular weight of 34 kDa, Tetra-Ubiquitin chains are composed of four Ubiquitin monomers that are covalently linked through isopeptide bonds, which typically form between a lysine residue of one Ubiquitin molecule and the C-terminal glycine residue of another Ubiquitin molecule (1). Each human Ubiquitin monomer is 76 amino acids (aa) in length and shares 96% and 100% aa identity with yeast and mouse Ubiquitin, respectively (2). Seven of the 76 aa in Ubiquitin are lysine residues that can participate in poly-Ubiquitin chain formation. Linkage through specific lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking, and other cellular processes (3-8).

This linear Ubiquitin fusion protein is resistant to the activity of deubiquitinatingenzymes (DUB"s) that cleave the peptide linkage between adjacent Ubiquitin molecules. Ubiquitin is not expressed directly as free Ubiquitin, but rather as linear fusions either to itself or to certain ribosomal protein subunits. These Ubiquitin-fusion precursors are proteolyzed by DUB"s at the appropriate junction points to yield active Ubiquitin monomers with C-termini ending in GG. There are likely several intracellular DUB"s which perform this essential processing role. This protein may be useful in analyzing interactions between linear Ubiquitin and proteins that contain Ubiquitin-associated domains (UBA"s) or Ubiquitin-interacting motifs (UIM"s).

References
  1. Scheffner, M. et al. (1995) Nature 373:81.
  2. Sharp, P.M. & W.-H. Li (1987) Trends Ecol. Evol. 2:328.
  3. Behrends, C. & J.W. Harper (2011) Nat. Struct. Mol. Biol. 18:520.
  4. Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
  5. Henry, A.G. et al. (2012) Dev. Cell 23:519.
  6. Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
  7. Wei, W. et al. (2004) Nature 428:194.
  8. Zhang, J. et al. (2012) J. Biol. Chem. 287:28646.
Entrez Gene IDs
7314 (Human)
Alternate Names
HEL-S-50; TetraUbiquitin; Tetra-Ubiquitin; Ub4; UBB; ubiquitin B

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Recombinant Enzymes

Recombinant Human His6-USP7 Protein, CF

E-519
4Citations
3Reviews
EnzAct

Recombinant Human His6UBE2S Protein, CF

E2-690
2Citations
EnzAct

Recombinant Human Isopeptidase T/USP5 Protein, CF

E-322
3Citations
EnzAct

Recombinant Human STAM-1 Protein, CF

E-550
2Citations
EnzAct

Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF

E-305
83Citations
2Reviews
EnzAct

Recombinant Human His6-UBE2N/UBE2V2 Complex Protein, CF

E2-666
1Citations
BA,BA

Recombinant Human His6-UAF1 Protein, CF

E-566
1Citations
BA

Recombinant Human UCH-L3 Protein, CF

E-325
3Citations
EnzAct

Recombinant Human UBE2K/E2-25K Protein, CF

E2-603
EnzAct

Reconstitution Buffers

Reconstitution Buffer 1 (PBS)

RB01
2Reviews

Reviews for Recombinant Human Linear Tetra-Ub Non-hydrolyzable, CF

There are currently no reviews for this product. Be the first toreview Recombinant Human Linear Tetra-Ub Non-hydrolyzable, CF and earn rewards!

Have you used Recombinant Human Linear Tetra-Ub Non-hydrolyzable, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review
Boston Biochem2011年,波士顿生物化学被TECHNE Corporation的 R&D Systems收购,R&D Systems是细胞生物学试剂的领先供应商,其拥有20多年的经验和独特的产品制造模式,可确保严格的质量控制以及产品中最高的一致性和性能。  凭借R&D System在销售,分销和物流方面的专业知识,Boston Biochem现在专注于新产品开发。在蛋白质纯化,表征,修饰和分析开发方面的广泛专业知识以及与UPP研究社区的紧密联系使我们能够继续提供最全面的产品范围。我们的收购也使我们有机会共同开发R&D Systems的互补抗体,ELISA和试剂盒以及Tocris Bioscience的互补抑制剂和底物。波士顿生物化学的所有专用工具将继续在内部进行开发,生产和表征,并致力于提供可负担性和卓越的产品。  2014年,Techne成为Bio-Techne,并与Boston Biochem和R&D Systems和Tocris的其他核心品牌一起为研究人员提供了广泛的产品和服务。随着我们成为波士顿生物化学品牌-Bio-Techne品牌,您可以期望我们提供与您相同的泛素专业知识和质量。