快报
欢迎光临蚂蚁淘在线！ 请登录 免费注册
商品
品牌
文章
热门搜索：
快报
品牌首页 品牌新闻 品牌简介 产品中心 品牌咨询 联系我们
品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
蚂蚁淘在线 / 品牌中心 / Boston Biochem / 波士顿生物化学/重组T。板栗His6-MBP-PINK1蛋白，CF/AP-180-100

波士顿生物化学/重组T。板栗His6-MBP-PINK1蛋白，CF/AP-180-100

价格
￥6980.00
货号：AP-180-100
浏览量：110
品牌：Boston Biochem
服务
全国联保
正品保证
正规发票
签订合同
商品描述
Product Details
Citations (1)
FAQs
Reviews
SummaryProduct DatasheetsCarrier FreeReconstitution CalculatorBackgroundRelated Research Areas

Recombinant T. castaneum His6-MBP-PINK1 Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity

Reaction conditions will need to be optimized for each specific application. We recommend an initial PINK1 concentration of 0.5-2 µM for the phosphorylation of recombinant Parkin, Ubiquitin, or Polyubiquitin chains.

Source
E. coli-derived t. castaneum PINK1 proteinContains an N-terminal MBP and 6-His tag
Accession #
 XP_968367.1
Predicted Molecular Mass
108 kDa

Product Datasheets

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AP-180

FormulationX mg/ml (X μM) in 20 mM HEPES pH 7.6, 50 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
ShippingThe product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: PINK1

Serine/Threonine kinase PINK1 (PTEN-induced putative kinase protein 1) plays a critical role in preventing mitochondrial dysfunction during cellular stress. PINK is translated in the cytosol, then translocated to the outer mitochondrial membrane where it is rapidly cleaved and degraded as a part of normal mitochondrial function. In damaged (depolarized) mitochondria PINK becomes stabilized and accumulates, resulting in the subsequent phosphorylation of numerous proteins on the mitochondrial surface including Mfn2. Ultimately PARK2 (E3 Ubiquitin Ligase Parkin) is recruited to the damaged mitochondria where it is activated by 1) PINK-mediated phosphorylation of PARK2 at serine 65, and 2) PARK2 interaction with phosphorylated Ubiquitin (also phosphorylated by PINK on serine 65). This signaling cascade is critical for clearing the damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PARK2.

Recombinant human PINK1 is not active in vitro, while this protein from the Red Flour Beetle (Tribolium castaneum) effectively phosphorylates recombinant Parkin, mono-Ubiquitin, and poly-Ubiquitin chains. It specifically phosphorylates both Parkin and Ubiquitin at serine 65. This recombinant protein contains N-terminal 6-His and MBP tags.

References
  1. Kane L.A., et al. (2014) J. Cell Biol. 205: 143
  2. Matsuda N., et al. (2010) J. Cell Biol. 189: 211
  3. Vives-Bauza C., et al. (2010) Proc. Natl. Acad. Sci. 107: 378
  4. Wauer T., et al. (2015) EMBO J. 34: 307
Long Name
PTEN-induced Putative Kinase 1
Entrez Gene IDs
65018 (Human); 68943 (Mouse); 298575 (Rat); 3925520 (T. castaneum)
Alternate Names
BRPK; EC 2.7.11.1; FLJ27236; PARK6; Parkinson disease (autosomal recessive) 6; PINK1; protein kinase BRPK; PTEN Induced Kinase 1; PTEN induced putative kinase 1; PTEN-induced putative kinase protein 1; serine/threonine-protein kinase PINK1, mitochondrial

Citation for Recombinant T. castaneum His6-MBP-PINK1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.

1Citation: Showing 1 - 1

  1. Analysis of neuronal phosphoproteome reveals PINK1 regulation of BAD function and cell deathAuthors: H Wan, B Tang, X Liao, Q Zeng, Z Zhang, L LiaoCell Death Differ., 2017;0(0):.Applications: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant T. castaneum His6-MBP-PINK1 Protein, CF

There are currently no reviews for this product. Be the first toreview Recombinant T. castaneum His6-MBP-PINK1 Protein, CF and earn rewards!

Have you used Recombinant T. castaneum His6-MBP-PINK1 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review
Boston Biochem2011年，波士顿生物化学被TECHNE Corporation的 R＆D Systems收购，R＆D Systems是细胞生物学试剂的领先供应商，其拥有20多年的经验和独特的产品制造模式，可确保严格的质量控制以及产品中最高的一致性和性能。  凭借R＆D System在销售，分销和物流方面的专业知识，Boston Biochem现在专注于新产品开发。在蛋白质纯化，表征，修饰和分析开发方面的广泛专业知识以及与UPP研究社区的紧密联系使我们能够继续提供最全面的产品范围。我们的收购也使我们有机会共同开发R＆D Systems的互补抗体，ELISA和试剂盒以及Tocris Bioscience的互补抑制剂和底物。波士顿生物化学的所有专用工具将继续在内部进行开发，生产和表征，并致力于提供可负担性和卓越的产品。  2014年，Techne成为Bio-Techne，并与Boston Biochem和R＆D Systems和Tocris的其他核心品牌一起为研究人员提供了广泛的产品和服务。随着我们成为波士顿生物化学品牌-Bio-Techne品牌，您可以期望我们提供与您相同的泛素专业知识和质量。