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蚂蚁淘在线 / 品牌中心 / Boston Biochem / 波士顿生物化学/重组人His6-USP22蛋白，CF/E-608-050

波士顿生物化学/重组人His6-USP22蛋白，CF/E-608-050

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￥5380.00
货号：E-608-050
浏览量：114
品牌：Boston Biochem
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SummaryProduct DatasheetsCarrier FreeReconstitution CalculatorBackgroundRelated Research Areas

Recombinant Human His6-USP22 Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant human USP22 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial USP22 concentration of 20-100 nM when using Ubiquitin-AMC or Ubiquitin-Rhodamine (U-550, U-555) substrates. Note: Recombinant USP22 is not active toward poly-Ubiquitin chains, Ubiquitinated histones and other Ubiquitinated protein substrates in the absence of adapter proteins such as ATXN7L or ENY2.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP22 proteinContains an N-terminal 6-His tag
Accession #
Q9UPT9
Predicted Molecular Mass
59 kDa

Product Datasheets

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-608

FormulationX mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, and 2 mM TCEP
ShippingThe product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: USP22

Ubiquitin carboxyl-terminal hydrolase 22 (USP22) is a specialized cysteine protease with a predicted molecular weight of 60 kDa.USP22 is a member of the peptidase C19 family (UBP8 subfamily) and the human protein shares 98% amino acid sequence identity with its mouse ortholog.USP22 is a member of the SAGA (Spt-Ada-Gcn5 acetyltransferase) complex, a 2 MDa protein machine that mediates the acetylation and deubiquitination of histones as well as non-histone substrates.Within SAGA, USP22 binds directly with ATXN7L3 and provides the activity required to deubiquitinate mono-Ubiquitinated histone H2B.Recent findings suggest USP27X and USP51, which function independently of SAGA, may compete with USP22 for ATXN7L3 and ENY2 binding, and that imbalances in these activities may contribute to human diseases including cancer.

References
  1. Atanassov B.S. et al. (2016) Mol. Cell 62:558.
  2. Atanassov B.S. et al. (2011) FEBS Lett. 585:2016.
  3. Atanassov B.S. & Dent S.R. (2016) personal communication.
  4. Koutelou E. et al. (2010) Curr Opin Cell Biol. 22:374.
  5. Lang G. et al. (2011) Mol Cell Biol. 31:3734.
  6. Zhang X.Y. et al. (2008) Mol. Cell 29:102.
  7. Zhao Y. et al. (2008) Mol. Cell 29:92.
Long Name
Ubiquitin Specific Protease 22
Entrez Gene IDs
23326 (Human); 216825 (Mouse); 303201 (Rat)
Alternate Names
Deubiquitinating enzyme 22; EC 3.1.2.15; EC 3.4.19.12; KIAA1063ubiquitin carboxyl-terminal hydrolase 22; KIAA1064; ubiquitin specific peptidase 22; ubiquitin specific peptidase 3-like; ubiquitin specific protease 22; Ubiquitin Thioesterase 22; Ubiquitin thiolesterase 22; ubiquitin-specific processing protease 22; Ubiquitin-specific-processing protease 22; USP22; USP3L

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Boston Biochem2011年，波士顿生物化学被TECHNE Corporation的 R＆D Systems收购，R＆D Systems是细胞生物学试剂的领先供应商，其拥有20多年的经验和独特的产品制造模式，可确保严格的质量控制以及产品中最高的一致性和性能。  凭借R＆D System在销售，分销和物流方面的专业知识，Boston Biochem现在专注于新产品开发。在蛋白质纯化，表征，修饰和分析开发方面的广泛专业知识以及与UPP研究社区的紧密联系使我们能够继续提供最全面的产品范围。我们的收购也使我们有机会共同开发R＆D Systems的互补抗体，ELISA和试剂盒以及Tocris Bioscience的互补抑制剂和底物。波士顿生物化学的所有专用工具将继续在内部进行开发，生产和表征，并致力于提供可负担性和卓越的产品。  2014年，Techne成为Bio-Techne，并与Boston Biochem和R＆D Systems和Tocris的其他核心品牌一起为研究人员提供了广泛的产品和服务。随着我们成为波士顿生物化学品牌-Bio-Techne品牌，您可以期望我们提供与您相同的泛素专业知识和质量。