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Applied Biological Materials/Cas9 Nuclease Non-Viral Vector/10 μg/85.00

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面议
货号:85.00
浏览量:127
品牌:abmgood
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商品描述
Specifications
Specifications
Description

This Cas9 nuclease can be used with a target-specific sgRNA to bind DNA and create a double-stranded cut. It is provided in a non-viral vector (plasmid) for quick and easy transfection. Transfection will result in transient expression of Cas9, leading to reduced chances of off-target editing. The Cas9 provided in this non-viral vector is spCas9 from Streptococcus pyogenes.

SKUK095
SystemNon-Viral Vector
Product Applications
  • Direct non-viral plasmid transfection for immediate expression.
  • Transient sgRNA expression reduces the chance of off-target effects.
Vector Map

pNV-EF1a-Cas9-2A-GFP

Unit quantity10 μg
PromoterEF1a
Cas TypeNuclease
Cas OriginspCas9
Cas9 ExpressionConstitutive Cas9
Note

abm"s sgRNAs are designed for use with Cas9 Nuclease only. Please inquire for custom sgRNAs for purposes other than genome editing (e.g. CRISPRa). For CRISPR-Cas9 system applications in genome regulation, please see our comprehensive collection of Cas9 mutant proteins (Nickase, Null mutant).

Shipping Conditions

Shipped at ambient temperature.

Bacterial SelectionAmpicillin
FormatPlasmid
AppearanceLiquid
Quality ControlRestriction Enzyme Digest and Sequencing
Shelf Life1 year (when at -20°C or below in a non-frost free freezer)
CautionThis product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Documents
Documents
Supporting Protocol
    MSDS
      QC
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          FAQs
          FAQs
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          References
          References
          There are no references for this product yet!
          Applied Biological Materials使用我们的CRISPR工具包,以前所未有的特异性编辑,标记,沉默或激活基因组的任何部分。立即使用CRISPR增强自身能力,使基因组成为您的游乐场!从sgRNA设计到筛选和验证,abm拥有CRISPR实验所有步骤所需的一切。或者,如果您愿意,我们的专家科学家可以为您执行整个CRISPR敲除程序。