One tube, one reaction – No stopper solution or multiple handling steps.  Reverse transcribe RNA to high-quality cDNA, including removal of contaminating genomic DNA, in under 30 minutes using 5X All-In-One RT MasterMix with AccuRT.

abm’s 5X All-in-One RT MasterMix with AccuRT offers a convenient, complete system for eliminating  contaminating gDNA in RNA samples and rapid synthesis of ready-to-use cDNA for downstream applications.

Key Features

• Efficient digestion of ss/dsDNA• Maximal RNA protection – No harsh extraction steps required• One tube, one reaction – No stopper solution or multiple handling steps• Streamlined protocol suitable for high-throughput applications• Simple setup for any RNA template• High reproducibility and cDNA yield

Note: For no-RT control, use a small aliquot of 5X All-In-One RT MasterMix with AccuRT (5 μl per no-RT sample you wish to include) and heat-inactivate all enzymes in the mastermix at 95°C for 5 min.

Store all components at -25°C to -15°C in a non-defrost cycle freezer. All components are stable for up to one (1) year from the date of shipping, if stored and handled properly.