Human CD95 (APO-1/FAS) is a type I cell surface glycoprotein that is strongly upregulated on activated T cells, B cells, NK cells and thymocytes (1). CD95 plays an important role in programmed cell death or apoptosis (2). Apoptosis appears to be a mechanism for regulating the immune response (3, 4).

Molecular Structure: A soluble fusion protein consisting of the mature extracellular (159 aa) domain of human CD95 fused to a linker (4 aa) and human IgG1 Fc + hinge (232 aa), with a (non glycosylated) predicted monomeric molecular weight of 44.3 kd.

CD95(EC):

rlssksvnaqvtdinskglelrktvttvetqnleglhhdgqfchkpcppgerkardctvngdepdcvpcqegkeytdkahfsskcrrcrlcdeghgleveinctrtqntkcrckpnffcnstvcehcdpctkcehgiikectltsntkckeegsrsnlg

linker: gtyv

Human Ig Fc + hinge:

eprscdkthtcppcpapellggpsvflfppkpkdtlmisrtpevtcvvvdvshedpevkfnwyvdgvevhnaktkpreeqynstyrvvsvltvlhqdwlngkeykckvsnkalpapiektiskakgqprepqvytlppsrdeltknqvsltclvkgfypsdiavewesngqpennykttppvldsdgsfflyskltvdksrwqqgnvfscsvmhealhnhytqkslslspgk

Transfectant Cell Line: CHO

Functional Application: CD95-huIg fusion protein inhibits CD178 mediated apoptosis of Jurkat cells.

References:

1. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 1142-1148.

2. S. Nagata & P. Golstein (1995) Science 267: 1449-1456.

3. S. Nagata & T. Suda (1995) Immunol Today 16: 39-43.

4. D.H. Lynch, F. Ramsdell & M.R. Alderson (1995) Immunol Today 16: 569-574.

5. H Wajant, (2003) Essays Biochem 39:53-71.

6. A. Ackery, M G Fehlings, (2006) J Neurotrauma 23(5): 604-616.

Ancell抗CD105异花青素人CD105（endoglin）是一种同型二聚体膜糖蛋白（1），实际上局限于内皮细胞，并且存在于一些白血病细胞和少量骨髓细胞中。CD105 以高亲和力与TGF- b 1和b 3结合（4）。同种型：鼠IgG1 kappa免疫原：人B谱系白血病细胞的膜制备特异性：抗体SN6识别分子量约为160 kd的人CD105同型二聚体的细胞外表位。据报道它与非人类灵长类动物以及马和猪（11）CD105发生交叉反应。功能应用：抗体SN6将增强TGF-b1与表达CD105的白血病细胞的结合。  抗体SN6已用于鉴定间充质祖细胞群（8,9,10）。