The inducible costimulator CD278 (ICOS) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Secondary signaling through CD28 or CD278 results in discrete cytokine secretion profiles by the activated T cells.¹ Signaling by either molecule is effectively down regulated by CD152 (CTLA-4) engagement.² Human CD275 (ICOSL, GL50, B7-H2) is a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2), and has been shown to be the ligand for CD278 (ICOS).³ Two RNA splice variants exist for this molecule, differing only in the cytoplasmic domain. ⁴

Isotype: Murine IgG1 Kappa

Immunogen: Human HPB-MLT cells, recombinant human CD278(ICOS)-muIg

Specificity: Antibody ANC6C6 binds to recombinant and cell surface CD278(ICOS)

Functional Application: Antibody ANC6C6 blocks binding of Recombinant CD275-muIg to CD278. It blocks CD28-superagonist/CD275(LICOS) mediated T cell proliferation in vitro(7).

References:

1) Beier, K.C., R.A. Kroczek, et al. 2000, Eur J Immunol. 30(12):3707-3717.

2) Riley, J.L., C.H. June, et al. 2001, J. Immunol. 166: 4943-4948.

3) Ling, V., M. Collins, et al. 2000, J. Immunol. 164: 1653-1657.

4) Ling, V., M. Collins, et al. 2001, J. Immunol. 166: 7300-7308.

5) Tomoki Ito, Yong-Jun Liu, et al. (2008) Immunity 28: 870-880.

6) Hyung W Lim, Chang H Kim. (2007) J Immunol 179: 7448-7456.

7) S Weissmuller, Z Waibler, et al. (2012) Blood 119(26):6268-6277.

Ancell抗CD105异花青素人CD105（endoglin）是一种同型二聚体膜糖蛋白（1），实际上局限于内皮细胞，并且存在于一些白血病细胞和少量骨髓细胞中。CD105 以高亲和力与TGF- b 1和b 3结合（4）。同种型：鼠IgG1 kappa免疫原：人B谱系白血病细胞的膜制备特异性：抗体SN6识别分子量约为160 kd的人CD105同型二聚体的细胞外表位。据报道它与非人类灵长类动物以及马和猪（11）CD105发生交叉反应。功能应用：抗体SN6将增强TGF-b1与表达CD105的白血病细胞的结合。  抗体SN6已用于鉴定间充质祖细胞群（8,9,10）。