Human CD272 (BTLA, B and T Lymphocyte Attenuator) is a member of the Immunoglobulin superfamily and has sequence homology to PD-1 and CTLA4. It is expressed on T and B lymphocytes and other hemopoetic lineages. Engagement of this molecule by its ligand, CD270(HVEM, a TNF superfamily member) can down regulate activated T and B cell responses. CD272 levels on antigen specific CD8+ T cells have been reported to decrease in viral specific, but not melanoma specific activated lines (1). Polymorphism of the CD272 molecule has been linked to rheumatoid arthritis (3).

Antibodies ANC6E9 and ANC5A5(cat #372-020) each bind to a distinct epitope of CD272 and are suitable as a matched pair for EIA (see attachment).

Isotype: Murine IgG1k

Immunogen: Recombinant extracellular human CD272

Specificity: Antibody from clone ANC6E9 binds to CD272 on the cell surface of human PBMC in Flow cytometry, and to recombinant CD272-muIg (cat #542-020) in EIA.

Functional Application: Antibody ANC6E9 blocks binding of Biotinylated CD270(HVEM)-muIg(cat #531-030) to CD272-muIg in EIA.

References:

1) Derre’ L, DE Speiser, et al. (2010) J Clin Invest 120(1):157-167.

2) Otsuki N, Azuma M, et al. (2006) Biochem Biophys Res Com 344(4): 1121-1127.

3) Lin SC, Chan CH, et al. (2006) J Biomed Sci 13(6): 853-860.

4) Pasero C, D Olive, et al. (2009) Curr Mol Med 9(7): 911-927.

5) Gavrieli M, KM Murphy, et al. (2006) Adv Immunol 92: 152-185.

Ancell抗CD105异花青素人CD105（endoglin）是一种同型二聚体膜糖蛋白（1），实际上局限于内皮细胞，并且存在于一些白血病细胞和少量骨髓细胞中。CD105 以高亲和力与TGF- b 1和b 3结合（4）。同种型：鼠IgG1 kappa免疫原：人B谱系白血病细胞的膜制备特异性：抗体SN6识别分子量约为160 kd的人CD105同型二聚体的细胞外表位。据报道它与非人类灵长类动物以及马和猪（11）CD105发生交叉反应。功能应用：抗体SN6将增强TGF-b1与表达CD105的白血病细胞的结合。  抗体SN6已用于鉴定间充质祖细胞群（8,9,10）。