The human B cell activating factor (BAFF) and APRIL(a proliferation inducing ligand) are both type II molecules belonging to the TNF superfamily. They are expressed by non-B cells, and are down-regulated by mitogenic stimulation.² BAFF and APRIL bind to at least two receptors: TACI (transmembrane activator and CAML-interactor) and BCMA (B cell maturation antigen), both of which are restricted to B cells.^3,4 Ligation of these receptors with recombinant BAFF dramatically increases IgM production by peripheral blood B cells ¹. Cell surface BAFF can be cleaved to form a soluble trimeric molecule.²

Molecular Structure: A soluble molecule consisting of 151 (VQGPEET…c-term) aminoacids of the extracellular domain of human BAFF fused to the extracellular domain (167aa) of murine CD8 alpha, with a predicted monomeric weight of 38.5 kd. A portion of the extracellular region of BAFF was truncated to amino acid 135 to eliminate a potential protease cleavage site.

Transfectant Cell Line: CHO BAFF(trn)-muCD8 binds to human cell surface TACI/BCMA/BAFFR (8, 9) .

Functional Application: Human BAFF(trn)-muCD8 binds to human cell surface TACI/BCMA (8, 9).

References:

1) Schneider P., J. Tschopp, et al. J. Exp. Med. 1999, 189(11):1747-1756.

2) Shu, H.B., H. Johnson, W.H. Hui. J Leukoc Biol 1999, 65:680-683.

3) Marsters, S.A., A. Ashkenazi, et al. 2000, Curr Biol 10:785-788.

4) Xia, X., H. Hsu, et al. 2000, J Exp Med, 192(1): 137-143.

5) Thompson J.S., C. Ambrose, et al. Science 2001, 293: 2108-2111.

6) Roschke, V, T.S. Migone, et al. J Immunol. 2002, 169: 4314-4321.

7) MacLennan, C.M., C.G. Vinuesa, 2002, Immunity 17:235-238.

8) Landau DA, P Cacoub, et al. (2009) Ann Rheum Dis 68(3): 337-44. PMID: 18434450.

9) Moreaux J, K Tarte, et al. (2004) Blood 103:3148-3157. PMID: 15070697.

10. EY Moon, S Lee, et al. (2011) Intl Immunopharmacol 11(10): 1523-1533. https://doi.org/10.1016/j.intimp.2011.05.009

Ancell抗CD105异花青素人CD105（endoglin）是一种同型二聚体膜糖蛋白（1），实际上局限于内皮细胞，并且存在于一些白血病细胞和少量骨髓细胞中。CD105 以高亲和力与TGF- b 1和b 3结合（4）。同种型：鼠IgG1 kappa免疫原：人B谱系白血病细胞的膜制备特异性：抗体SN6识别分子量约为160 kd的人CD105同型二聚体的细胞外表位。据报道它与非人类灵长类动物以及马和猪（11）CD105发生交叉反应。功能应用：抗体SN6将增强TGF-b1与表达CD105的白血病细胞的结合。  抗体SN6已用于鉴定间充质祖细胞群（8,9,10）。