Human CD252 (CD134L, OX40 Ligand) is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells. CD252 binds to CD134(OX40) present on activated T cells, providing a costimulatory signal (1). This pathway seems to be more effective for costimulation of CD4+ helper T cells than for CD8+ effector T cells (4). Blockade of this interaction in mouse abrogated immunological effects in several models of inflammation and rejection (5,6,7,8).

Isotype: Murine IgG1 Kappa

Immunogen: Human HUVEC and Recombinant soluble human CD252

Specificity: Antibody ANC10G1 binds to CD252 on the cell surface of HUVEC in Flow cytometry, and blocks binding of recombinant CD134-muIg.

Functional Application: Antibody ANC10G1 blocks binding of Recombinant CD134 to CD134L on HUVEC.

References:

1) W. R. Godfrey, et al, (1994) J Exp Med 180: 757-763.

2)L.M. Higgins, et al, (1999) J Immunol 162: 486-493.

3) A.D. Weinberg, et al, (1999) J Immunol 162: 1818-1826.

4) V.Y. Taraban, et al, (2002) Eur J. Immunol. 32: 3617-3627.

5) V. Malmstrom, et al, (2001) J Immunol 166: 6972.

6) C. Nohara, et al, (2001) J Immunol 166: 2108-2115.

7) X. Yuan, et al, (2003) J Immunol 170: 2949-2955.

8) L. Tian, et al, (2002) Transplantation 74(1): 133-138.

9) T Uenotsuchi, M Furue, et al. (2006) Intl Immunol 18(12): 1637-1646.

Ancell抗CD105异花青素人CD105（endoglin）是一种同型二聚体膜糖蛋白（1），实际上局限于内皮细胞，并且存在于一些白血病细胞和少量骨髓细胞中。CD105 以高亲和力与TGF- b 1和b 3结合（4）。同种型：鼠IgG1 kappa免疫原：人B谱系白血病细胞的膜制备特异性：抗体SN6识别分子量约为160 kd的人CD105同型二聚体的细胞外表位。据报道它与非人类灵长类动物以及马和猪（11）CD105发生交叉反应。功能应用：抗体SN6将增强TGF-b1与表达CD105的白血病细胞的结合。  抗体SN6已用于鉴定间充质祖细胞群（8,9,10）。