Molecular Structure: A soluble fusion protein consisting of the extracellular (137aa) domain of human CD279(PD-1): (25)ldspdrpwnpptfspallvvtegdnatftcsfsntsesfvlnwyrmspsnqtdklaafpedrsqpgqdcrfrvtqlpngrdfhmsvvrarrndsgtylcgaislapkaqikeslraelrvterraevptahpspspr(161)

Linking amino acids: sagtr

murine IgG2a Fc +hinge: (133 aa) eprgptikpcppckcpapnllggpsvfifppkikdvlmislspivtcvvvdvseddpdvqiswfvnnvevhtaqtqthredynstlrvvsalpiqhqdwmsgkefkckvnnkdlpapiertiskpkgsvrapqvyvlpppeeemtkkqvtltcmvtdfmpediyvewtnngktelnykntepvldsdgsyfmysklrvekknwvernsyscsvvheglhnhhttksfsrtpgk

In SDS-PAGE, this protein runs at 52 kd under native, and 27 kd under reducing conditions.

Data suggest that the murine Ig Fc proximal portion of this construct may be prone to cleavage.

Transfectant Cell Line: CHO

INFORMATION Human CD279 (PD-1, Programmed death 1) is a 55 kd Ig superfamily member with similarity to CD28 and CD152(CTLA-4). It is expressed on activated T and B and myeloid cells, and engagement by its ligands PD-L1 (CD274, B7-H1) or PD-L2 (B7-DC) can inhibit proliferation and cytokine expression. In mice blockade of PD-1 ligand interaction has been used to augment T cell anti cancer responses (1,2,3).

Recombinant CD279 -muIg binds to recombinant CD274 and CD273 in EIA and cell surface CD273 on moDC in FACS. It is recognized by anti-CD279 mAb (cat #279-030; clone ANC4H6) in EIA.

References: . 1) MA Curran, Allison JP, et al. (2010) PNAS 107(9): 4275-80. 2) Mangsbo SM, TH Totterman, et al. (2010) J Immunotherapy 33(3):225. 3) Hirano F, L Chen, et al. (2005) Canc Res 65: 1089.

Ancell抗CD105异花青素人CD105（endoglin）是一种同型二聚体膜糖蛋白（1），实际上局限于内皮细胞，并且存在于一些白血病细胞和少量骨髓细胞中。CD105 以高亲和力与TGF- b 1和b 3结合（4）。同种型：鼠IgG1 kappa免疫原：人B谱系白血病细胞的膜制备特异性：抗体SN6识别分子量约为160 kd的人CD105同型二聚体的细胞外表位。据报道它与非人类灵长类动物以及马和猪（11）CD105发生交叉反应。功能应用：抗体SN6将增强TGF-b1与表达CD105的白血病细胞的结合。  抗体SN6已用于鉴定间充质祖细胞群（8,9,10）。