Human IgG1k from human myeloma plasma is used as a negative control(1,2) for work involving human IgG1 isotype antibodies or recombinant proteins.

The molecular weight of this myeloma derived protein has varied for some lots; running at about 135 kD in SDS-PAGE, compared to the expected 150 kD for normal human IgG1. This was due to a smaller heavy chain, which ran at approximately 40 kD when reduced.

References

1) J Shen, PS Low, et al. (2014) J Leuk Biol 96: 563-570. PMID: 25015955

2) C E Green, S I Simon, et al.(2003) Am J Physiol - Cell Physiol 284(3): C705-C717.

3) S Watanabe, S G Vasudevan, et al.(2015) J Virol 89(11): 5847-61.

Ancell抗CD105异花青素人CD105（endoglin）是一种同型二聚体膜糖蛋白（1），实际上局限于内皮细胞，并且存在于一些白血病细胞和少量骨髓细胞中。CD105 以高亲和力与TGF- b 1和b 3结合（4）。同种型：鼠IgG1 kappa免疫原：人B谱系白血病细胞的膜制备特异性：抗体SN6识别分子量约为160 kd的人CD105同型二聚体的细胞外表位。据报道它与非人类灵长类动物以及马和猪（11）CD105发生交叉反应。功能应用：抗体SN6将增强TGF-b1与表达CD105的白血病细胞的结合。  抗体SN6已用于鉴定间充质祖细胞群（8,9,10）。