Human CD32 exists in at least six isoforms originating from three different genes (Fc gamma RII A, B, and C) . The CD32 molecule is a low affinity receptor for immune complexed IgG and has signal-transducing capabilities involved with humoral and cell-mediated immune responses.

Isotype: Murine IgG1k

Immunogen: Human K562 tumor cells and L cells transfected with human Fc gamma RII

Specificity: Antibody 7.3 reacts with a domain 2 epitope of all CD32 isoforms (1). It exhibits preferential binding to Fc gRIIb (inhibitory) isoform transfectants(2, 5).

Functional Application: Intact and F(ab‘)₂ 7.3 antibody blocks immune complex binding (4). Incubation with anti-CD32 F(ab‘)2 inhibits Fibrocyte differentiation(6), and inhibits pro inflammatory cytokine secretion by SLE patient derived monocytes (7).

References:

1. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 823-836.

2. Liu Y, L Pricop, et al. (2005) J Leukocyte Biol 77: 767-776 PMID:15703199

3. S. von Gunten, H.U. Simon, et al. (2006) Blood 108(13): 4255-425

4. Laborde EA, M Vulcano, et al. (2007) J Immunol 179(1): 673-681.

5. Ierino FL, P M Hogarth, et al. (1993) JImmunol 150(5):1794-1803.

6. Pilling D, NM Tucker, RH Gomer (2006) J Leuk Biol 79(6): 1242-1251.

7. Thanei S, M Trendelenburg (2016) J Immunology 196: 2063–2074. PMID: 26829984

Ancell抗CD105异花青素人CD105（endoglin）是一种同型二聚体膜糖蛋白（1），实际上局限于内皮细胞，并且存在于一些白血病细胞和少量骨髓细胞中。CD105 以高亲和力与TGF- b 1和b 3结合（4）。同种型：鼠IgG1 kappa免疫原：人B谱系白血病细胞的膜制备特异性：抗体SN6识别分子量约为160 kd的人CD105同型二聚体的细胞外表位。据报道它与非人类灵长类动物以及马和猪（11）CD105发生交叉反应。功能应用：抗体SN6将增强TGF-b1与表达CD105的白血病细胞的结合。  抗体SN6已用于鉴定间充质祖细胞群（8,9,10）。