产品概述 Abcam s VCAM1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VCAM1 in serum, plasma and cell culture supernatants.This assay employs an antibody specific for Human VCAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and VCAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VCAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VCAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.Get results in 90 minutes with Human VCAM1 ELISA Kit (CD106) (ab187393) from our SimpleStep ELISA range. 1 x 30mlBiotinylated anti-Human VCAM1 2 vialsRecombinant Human VCAM1 Standard (lyophilized) 2 vialsStop Solution 1 x 8mlTMB One-Step Substrate Reagent 1 x 12mlVCAM1 Microplate (12 x 8 wells) 1 unit Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 组织特异性 Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue. Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion. 发表研究结果有使用 ab100661？请让我们知道，以便我们可以引用本数据表中的参考文章。 ab100661 被引用在 2 文献中. Shi J et al. Curcumin pretreatment protects against PM2.5-induced oxidized low-density lipoprotein-mediated oxidative stress and inflammation in human microvascular endothelial cells. Mol Med Rep 16:2588-2594 (2017).PubMed: 28713935 Strøm Halden TA et al. Short-term efficacy and safety of sitagliptin treatment in long-term stable renal recipients with new-onset diabetes after transplantation. Nephrol Dial Transplant 29:926-33 (2014).PubMed: 24452849 Ab46075 uses two different mouse monoclonal antibodies. However, the difference in the kits is that the capture antibody for ab100661 is a monoclonal mouse and the detection antibody is a polyclonal sheep. I hope that you find this information helpful. Please feel free to contact us again should you have further questions about this antibody or any of the products in our catalogue.     Read MorePlease note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES For licensing inquiries, please contact partnerships@abcam.com

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