CD4重组抗体[EPR6855]_CD4抗体(ab133616)| Abcam中文官网-蚂蚁淘在线

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply Rabbit monoclonal [EPR6855] to CD4 Suitable for: Flow Cyt (Intra), ICC, WB, IHC-P Reacts with: Human

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

WB: THP-1 and HuT-78 cell lysates, human fetal thymus, tonsil and lymph node tissue lysates.IHC-P: Human tonsil, liver, spleen, thymoma and colon tissues.ICC: Human peripheral blood lymphocytes and THP-1 cells.Flow Cyt (intra): Human peripheral blood lymphocytes.
常规说明

This product is a recombinant monoclonal antibody, which offers several advantages including:- High batch-to-batch consistency and reproducibility- Improved sensitivity and specificity- Long-term security of supply- Animal-free productionFor more information see here.

Our RabMAb technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb patents.

We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated \'PUR\' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
存储溶液 pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS
The Abpromise guarantee

Abpromise™承诺保证使用ab133616于以下的经测试应用

\"应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

Flow Cyt (Intra) 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/5000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

For unpurified use at 1/1000 - 1/10000.

IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
序列相似性 Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
翻译后修饰 Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
细胞定位 Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
T cell differentiation antigen L3T4 antibody T cell OKT4 deficiency, included antibody T cell surface antigen T4/Leu 3 antibody T cell surface antigen T4/Leu3 antibody T cell surface glycoprotein CD4 antibody T-cell surface antigen T4/Leu-3 antibody T-cell surface glycoprotein CD4 antibody W3/25 antibody W3/25 antigen antibody see all

Human peripheral blood lymphocytes stained with unpurifiedab133616 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22 C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37 C. For experimentation, cells were treated with 50% methanol (-20 C) for 15 min at 4 C. Cells were then incubated with the antibody (unpurified ab133616,1/100 dilution) for 30 min at 4 C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H L) (ab150077) at 1/2000 dilution for 30 min at 4 C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1 g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of 30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labellingCD4 with purified ab133616 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

All lanes : Anti-CD4 antibody [EPR6855] (ab133616) at 1/1000 dilution (unpurified)

Lane 1 : THP-1 cell lysate
Lane 2 : Human fetal thymus lysate
Lane 3 : Human tonsil lysate
Lane 4 : Human lymph node lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lane 1 : HRP labelled goat anti-rabbit at 1/2000 dilution
Lanes 2-4 : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 51 kDa
Observed band size: 51 kDa

Immunocytochemistry analysis of THP-1 (Human monocytic leukemia monocyte) labeling CD4 with purified ab133616 at 1/100 dilution. Cells were fixed with 100% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.30 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody.
Immunocytochemistry - Anti-CD4 antibody [EPR6855] (ab133616)Baratella et al PLoS Negl Trop Dis. 2017 Jan; 11(1): e0005285. Published online 2017 Jan 17. doi: 10.1371/journal.pntd.0005285

HBZ is preferentially expressed in CD4+ T cells of HAM/TSP patient PH1624

Confocal microscopy analysis of PBMC from HAM/TSP patient PH1624. (A) co-staining with the 4D4-F3 anti-HBZ mAb followed by Alexa Fluor 546-conjugated goat anti-mouse IgG1 antibody (red) and with the anti-CD4 mAb followed by Alexa Fluor 488-conjugated goat-anti-rabbit IgG antibody (green); upper panels, extended field; lower panels, enlarged field focused on the single cell depicted in the square of the left upper panel and positive for both CD4 and HBZ.

CD4 was detected using ab133616 at 1/100 dilution.

From Figure 6A of Baratella et al.

Baratelle et alPLoS Negl Trop Dis. 2017 Jan; 11(1): e0005285.Published online 2017 Jan 17.doi:10.1371/journal.pntd.0005285

Reproduced underCreative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Different batches of ab133616 were tested on THP-1 (Human monocytic leukemia monocyte) lysate at 1.0 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 51 kDa.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thymoma tissue labelling CD4 with purified ab133616 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling CD4 with ab133616 at a dilution of 1/100.