Product Description

Recombinant 2019-nCoV NSP1 is produced by our E.coli expression system and the target gene encoding Met1-Gly180 is expressed with a 6His tag at the C-terminus.Background:The Severe Acute Respiratory Syndrome (SARS) Coronavirus (CoV) is an enveloped, positive-stranded RNA viruses that can cause a severe respiratory disease. Its genome consists of a ∼30 kb linear, non-segmented, capped, polycistronic, polyadenylated RNA molecule, the first two-third of which is directly translated into two large polyproteins. These two polypeptides are processed into 16 non-structural proteins (nsps), forming the replicase complex, which is active in the cytoplasm in close association with cellular membranes. Nsp1 was proved to be able to suppress host gene expression by promoting host mRNA degradation and was involved in cellular chemokine deregulation. This virus evades the host innate immune response in part through the expression of its non-structural protein (nsp) 1, which inhibits both host gene expression and virus- and interferon (IFN)-dependent signaling. Thus, nsp1 is a promising target for drugs, as inhibition of nsp1 would make SARS-CoV more susceptible to the host antiviral defenses.Formulation:Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.5.Purity:> 85 % as determined by reducing SDS-PAGE.Endotoxin:< 1.0 EU per µg as determined by the LAL method.Shipping:This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C. Avoid freeze-thaw cycles.

More Details:Sequence:Met1-Gly180Fusion Tag:C-6HisAccession:YP_009725297.1Species:VirusExpression System:E. coliMol Mass:22.7 kDaAP Mol Mass:25 kDaSynonyms:SARS-CoV 2 nsp1; SARS-CoV 2 Leader proteinDatasheet

FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.Manufactured by: Elabscience

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Iwai-Chem我们的大肠杆菌表达系统产生了重组的2019-nCoV 3C样蛋白酶，编码Ser1-Gln306的靶基因在N端表达了6His标签。背景：病毒主要蛋白酶（M pro，也称为3CL pro），控制冠状病毒复制复合物的活性。它起半胱氨酸蛋白酶的作用，参与病毒前体多蛋白的蛋白水解切割成冠状病毒复制所需的一系列功能蛋白，被认为是设计抗SARS药物的诱人靶标。配方：  以0.2μm的PBS，1mM EDTA，10％甘油，pH 7.4的过滤溶液形式提供。纯度：  > 95％（通过还原SDS-PAGE确定）。内毒素： 通过LAL方法测定，每µg <1.0 EU。运输：  本产品以液体形式提供。它以蓝色冰/凝胶包装在冷冻温度下运输。收到后立即将其储存在<-20°C下。避免冻融循环。