Description

ProteintagsareproteinorpeptidesequenceslocatedeitherontheC-orN-terminalofthetargetprotein,whichfacilitatesoneorseveralofthefollowingcharacteristics:solubility,detection,purification,localizationandexpression.Greenfluorescenceprotein(GFP)isaproteincomposedof238aminoacidresidues(26.9kDa)derivedfromtheJellyfishAequoreavictoria,whichemitsgreenlight(emissionpeakat509nm)whenexcitedbybluelight(excitationpeakat395nm).GFPhasbecomeaninvaluabletoolincellBIOLOGyresearch,sinceitsintrinsicfluorescencecanbevisualizedinlivingcells.EGFPcontainsthedouble-amino-acidsubstitutionsPhe-64toLeuandSer-65toThr(previouslypublishedasGFPmut1).IncontrasttowtGFP,EGFPhasasingle,strong,red-shiftedexcitationpeakat488nm.GFPmut1fluoresces35-foldmoreintenselythanwtGFPwhenexcitedat488nm,duetoanincreaseinitsextinctioncoefficient(Em).

SpecSheetE-AB-20024(PDFFile)

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FORRESEARCHUSEONLY,NOTFORUSEINDIAGNOSTICPROCEDURES.

*RemarkIcon:

WB=WesternBlotting,IP=Immunoprecipitation,IF=Immunofluorescence,IHC=Immunohistochemistory,IHC-p=ImmunohistochemistoryParaffin,FCM=FlowCytometry,CH=ChIPAssay

Manufacturedby:Elabscience Iwai-Chem我们的大肠杆菌表达系统产生了重组的2019-nCoV 3C样蛋白酶，编码Ser1-Gln306的靶基因在N端表达了6His标签。背景：病毒主要蛋白酶（M pro，也称为3CL pro），控制冠状病毒复制复合物的活性。它起半胱氨酸蛋白酶的作用，参与病毒前体多蛋白的蛋白水解切割成冠状病毒复制所需的一系列功能蛋白，被认为是设计抗SARS药物的诱人靶标。配方：  以0.2μm的PBS，1mM EDTA，10％甘油，pH 7.4的过滤溶液形式提供。纯度：  > 95％（通过还原SDS-PAGE确定）。内毒素： 通过LAL方法测定，每µg <1.0 EU。运输：  本产品以液体形式提供。它以蓝色冰/凝胶包装在冷冻温度下运输。收到后立即将其储存在<-20°C下。避免冻融循环。