Description	Enzyme Immunoassay for the determination of Chicken TNF-α.
Method Type	Sandwich-Ab
Sensitivity	18.75 pg/mL
Assay Range	31.25~2000 pg/mL
Other Name	DIF, TNF-alpha, TNFA, TNFSF2
Principle	This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Chicken TNF-α. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Chicken TNF-α and Avidin- Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Chicken TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme- substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Chicken TNF-α. The concentration of Chicken TNF-α in samples can be calculated by comparing the OD of the samples with the standard curve.
Intended Use	This ELISA kit can be applied to the in vitro quantitative determination of Chicken TNF-α concentrations in serum, plasma and other biological fluids.
Specificity	This kit recognizes some recombinant and natural Chicken TNF-α. No significant cross-reactivity or interference was observed.
Sample Type	Serum, plasma and other biological fluids
Kit Components	Micro ELISA Plate, 8 x 12 well strips Reference Standard, 2 vials Concentrated Biotinylated Detection Ab(100x), 120 µL Concentrated HRP Conjugate(100x), 120 µL Reference Standard and Sample Diluent, 20 mL Biotinylated Detection Ab Diluent, 14 mL HRP Conjugate Diluent, 14 mL Concentrated Wash Buffer (25x), 30 mL Substrate Reagent, 10 mL Stop Solution, 10 mL Plate Sealer, 5 pieces Product Manual Certificate of Analysis
Other supplies required	Microplate reader with 450nm wavelength filter High-precision transferpettor, EP tubes and disposable pipette tips 37°C Incubator Deionized or distilled water Absorbent paper Loading slot for Wash Buffer
Storage	All reagents in this kit should be stored in accordance with the information on product labels. Unused wells should be returned to the foil pouch with the desiccant pack and resealed until air-tight. The Substrate Reagent should not be kept at -20°C. Avoid exposing reagents to strong light during storage or the incubation process. Caps of reagents should be kept tightly sealed to prevent evaporation and microbial contamination. Erroneous results may occur if reagents are not stored as advised above.

Data Sheet E-EL-Ch0215 (PDF File)

Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. Vials are prone to fall over. Use caution, especially when the lid is off .

FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Manufactured by : Wuhan Elabscience Biotechnology Co. , Ltd

 

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Iwai-Chem我们的大肠杆菌表达系统产生了重组的2019-nCoV 3C样蛋白酶，编码Ser1-Gln306的靶基因在N端表达了6His标签。背景：病毒主要蛋白酶（M pro，也称为3CL pro），控制冠状病毒复制复合物的活性。它起半胱氨酸蛋白酶的作用，参与病毒前体多蛋白的蛋白水解切割成冠状病毒复制所需的一系列功能蛋白，被认为是设计抗SARS药物的诱人靶标。配方：  以0.2μm的PBS，1mM EDTA，10％甘油，pH 7.4的过滤溶液形式提供。纯度：  > 95％（通过还原SDS-PAGE确定）。内毒素： 通过LAL方法测定，每µg <1.0 EU。运输：  本产品以液体形式提供。它以蓝色冰/凝胶包装在冷冻温度下运输。收到后立即将其储存在<-20°C下。避免冻融循环。