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Selecting a blocking buffer that best suppresses non-specific signals is critical to obtaining clear, specific Western blot results. The protein-free version is completely chemically defined, has a higher sensitivity than other commonly used blocking agents, such as nonfat milk and is excellent for detecting phosphorylated proteins. The Blocking Buffer (Fish Gelatin) is a reagent of fish origin that is less likely to cross-react with mammalian antibodies than conventional blocking reagents.
Selecting a blocking buffer that best suppresses non-specific signals is critical to obtaining clear, specific Western blot results. The protein free version is completely chemically defined, has a higher sensitivity than other commonly used blocking agents, such as nonfat milk and is excellent for detecting phosphorylated proteins. The Blocking Buffer (Fish Gelatin) is a reagent of fish origin that is less likely to cross-react with mammalian antibodies than conventional blocking reagents.
To obtain the highest possible detection signal for Western blotting, use these products in combination with the Western BLoT HRP Substrate Series for HRP chemiluminescence.
