The Diversify PCR Random Mutagenesis Kit employs a nontoxic method for mutagenizing sequences of up to 4.0 kb in length. The kit broadly distributes all possible mutations without creating hot spots. By allowing you to manipulate mutagenic conditions, the Diversify PCR Random Mutagenesis Kit provides optimal mutagenesis of sequences over an exceptionally broad size range. Select mutation rates from two to eight mutations per 1,000 bp simply by varying the amounts of two key reagents—manganese (Mn²⁺) and dGTP (Cadwell and Joyce 1992; Leung, Chen, and Goeddel 1989).

The Diversify PCR Random Mutagenesis Kit contains our Titanium Taq PCR system to reliably amplify DNA fragments of up to 4.0 kb in length. This extended amplification range makes the Diversify kit ideal for mutating operons, plasmids, and sequences corresponding to large proteins, in addition to short DNA fragments. The kit also contains a rapid control reaction that allows the relative comparison of mutation rates in the control fragment in just two hours following PCR. In addition to the Titanium Taq polymerase mix, the Diversify PCR Random Mutagenesis Kit includes all of the reagents and buffers needed for mutagenesis.