This page describes a two-vector lentivirus format for inducible expression. Click the link to learn more about our all-in-one Lenti-X Tet-One inducible expression systems. Lenti-X Tet-On 3G inducible expression systems combine the broad cellular tropism of lentiviral gene transfer technology with the power and versatility of our state-of-the-art tetracycline-regulated gene expression system. The lentiviral Tet-On-3G system consists of two lentiviral vectors: a regulator vector that stably expresses the Tet-On 3G transactivator protein, and a response vector that contains the TRE3G promoter that controls the expression of your transgene. Also included are a control response vector for luciferase expression, and our premium Lenti-X Packaging Single Shots (VSV-G).

How it works

Doxycycline-controlled transactivators are fusion proteins that contain a TetR-derived DNA binding domain joined to three minimal transcription activation domains from VP16 (Tet-On 3G). With very high specificity, the transactivator recognizes and binds to a tetracycline response element (TRE) that is located in the P_TRE3G promoter of pLVX-TRE3G, into which you clone your gene of interest. Binding of the transactivator to the TRE promoter results in strong activation of the downstream gene. The Tet-On 3G system is the premium, third-generation version of the technology, with the tightest TRE promoter and most sensitive transactivator. TheTet-Onesystem uses the same components but assembled on a single vector.

In all Tet-On systems, gene expression is activated by the addition of Dox to the growth medium, while in Tet-Off systems, transcription is activated by its withdrawal.

High-titer lentiviral packaging

Our Lenti-X Packaging Single Shots (VSV-G), supplied with each system, generate superior titers compared to most commercially available packaging systems.