Strand-specific RNA-seq libraries for Illumina platforms from high-input total RNA

The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian provides a rapid solution for preparing indexed Illumina sequencing libraries from 100 ng–1 µg of mammalian total RNA. This kit combinesRiboGoneand SMART technologies, seamlessly incorporating efficient rRNA removal followed by strand-specific library generation in around 5 hours. Because rRNA can comprise ~90% or more of total RNA, effective depletion of rRNA prior to library generation can lower sequencing costs and improve mapping statistics. SMART cDNA synthesis technology followed by PCR complete a streamlined process that performs strand information and eliminates downstream library prep by incorporating indexes and adapters during the reverse transcription and amplification steps. These high-input kits contain 12 reverse primers and up to 8 forward primers that allow for multiplexing of up to 96 separate libraries in a high-throughput format. The specific primers you receive will depend on your particular kit. The entire protocol—from rRNA depletion through creation of Illumina-specific, indexed RNA-seq libraries—takes only about 5 hours.

Alternate kits for other input amounts:

• For 250 pg–10 ng of total RNA, we recommend the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian
• For 10–100 ng of total RNA, we recommend the SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian