品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
| Unit Definition | 1 unit is the enzyme activity that incorporates 1 nmol of [3H]-dTMP into acid-precipitable products in 10 minutes at +37 °C with poly(A)+ · d(pT)15 as template primer. |
|---|---|
| Application | First- and second-strand cDNA synthesis and synthesis of first strand cDNA |
| Dideoxy DNA sequencing | |
| Primer extension | |
| RNA sequencing | |
| 3′-end labeling of DNA fragments |
AMV Reverse Transcriptase (AMV RT) catalyzes the polymerization of DNA using template DNA, RNA or RNA:DNA hybrides. It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg²⁺ or Mn²⁺. The enzyme possesses an intrinsic RNase H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro.
