avivasysbio/Cell Stimulation Cocktail (OOTA00786)/OOTA00786

价格
面议
货号:OOTA00786
浏览量:76
品牌:avivasysbio
服务
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商品描述
Product Format:
Liquid. 500X concentrate of Phorbol 12-Myristate 13-Acetate, Ionomycin, Brefeldin A, and Monensin in Ethanol
Application:
FC, FN
Additional Information:
Description:

Cell Stimulation Cocktail (500X) is a ready to use cocktail that can be used for polyclonal cell activation. It is composed of the phorbol ester, PMA (Phorbol 12-Myristate 13-Acetate), a calcium ionophore (Ionomycin), and the protein transport inhibitors Brefeldin A and Monensin. This Cell Stimulation Cocktail (500X) can be used to elicit cytokine production and retention in a variety of cell types allowing for detection of intracellular protein by flow cytometry.

Reconstitution and Storage:
-20C, protected from light and tightly sealed to prevent evaporation
Application Info:
Cell Stimulation Cocktail (500X) has been quality-tested by treatment of mouse splenocytes for 6 to 18 hours followed by intracellular staining and flow cytometric analysis of IL-2 or IFN-gamma. Cell Stimulation Cocktail is supplied as a 500X solution and should be used at 1X final concentration for cell stimulation. It is recommended to add the cocktail directly to the cell suspension at 2 uL/mL to obtain a 1X final concentration.
Datasheets/Manuals:
Printable datasheet for OOTA00786
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