avivasysbio/Cell Viability Dye Violet 540 (OOTA00664)/OOTA00664

价格
面议
货号:OOTA00664
浏览量:69
品牌:avivasysbio
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商品描述
Application:
FC
Additional Information:
Description: Cell Viability Dye Violet 540 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Cell Viability Dye Violet 540 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.
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Excitation Laser: Violet (405 nm)
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Emission: 537 nm
Reconstitution and Storage:
-20C protected from light and moisture
Application Info:
Cell Viability Dye Violet 540 has been quality-tested for flow cytometry using mouse bone marrow cells and can be used at 1 uL/mL of cell suspension. The concentration required for optimal performance should be determined empirically by investigator.Cell Viability Dye Violet 540 is excited by the violet (405 nm) laser line and has a peak emission of 540 nm that can be detected using a 525/50 band pass filter commonly used for detection of AmCyan.
Formulation:
1 uL/test in DMSO
Datasheets/Manuals:
Printable datasheet for OOTA00664
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