Predicted Species Reactivity:Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat, ZebrafishProduct Format:Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Clonality:PolyclonalHost:GoatApplication:WBReconstitution and Storage:Aliquot and store at -20C. Minimize freezing and thawing.Replacement Item:This antibody may replace item sc-28687 from Santa Cruz Biotechnology.Predicted Homology Based on Immunogen Sequence:Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 100%Complete computational species homology data:LEF1 Antibody (OAEB01737)Peptide Sequence:C-QHEQRKEQEPKRPHDatasheets/Manuals:Printable datasheet for anti-LEF1 antibody - OAEB01737Purity:Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.Application Info:Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot: Approx 48kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 44.2kDa according to NP_057353.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yasumoto et al, EMBO J. 2002 Jun 3;21(11):2703-14.; PMID: 12032083). Recommended concentration: 1-3ug/ml.Target Reference:* Radtke F, Clevers H.; Self-renewal and cancer of the gut: two sides of a coin.; Science. 2005 Mar 25;307(5717):1904-9. Review.; Pubmed 15790842Gene Symbol:LEF1Alias Symbols:LEF1, lymphoid enhancer-binding factor 1, HGNC:6551, DKFZp586H0919, TCF1ALPHA, lymphoid enhancer binding factor-1NCBI Gene Id:51176Protein Name:Lymphoid enhancer-binding factor 1Swissprot Id:Q9UJU2Protein Accession #:NP_057353.1Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express LEF1. 
RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express LEF1. 
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