Predicted Species Reactivity:DogApplication:ELISAKit Detection Method:Colorimetric, OD450 nmKit Linearity:| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 103-113% (n = 20) | 98-108% (n = 20) | 105-115% (n = 20) | 108-118% (n = 20) |
| EDTA plasma(n=5) | 97-106% (n = 20) | 107-117% (n = 20) | 106-116% (n = 20) | 104-114% (n = 20) |
| heparin plasma(n=5) | 105-114% (n = 20) | 81-81% (n = 20) | 107-117% (n = 20) | 114-114% (n = 20) |
Kit Principle:Aviva Systems Biology MMP3 ELISA Kit (Dog) (OKEH07291) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MMP3 captured in well.Kit Range:0.78 - 50ng/mLKit Recovery:Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99% (n = 20)Kit Reproducibility:Mean Intra-assay CV%: <=5.9% (n="">Mean Inter-assay CV%: <=8.6% (n="">Kit Component:| Component | Amount |
|---|
| MMP3 Microplate | 96 Wells (12 x 8 Well strips) |
| MMP3 Lyophilized Standard | 2 |
| 100X Biotinylated MMP3 Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Sample Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| TMB Substrate | 1 x 10 mL |
| Stop Solution | 1 x 10 mL |
Additional Information:Function: Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.:::Subcellular Location: Secreted extracellular space extracellular matrixReconstitution and Storage:Store as indicated in product manual.Sensitivity:0.21ng/mLDatasheets/Manuals:Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.Specificity:Natural and recombinant Dog Stromelysin-1Assay Info:Assay Methodology: Quantitative Sandwich ELISAGene Symbol:MMP3Alias Symbols:SL-1, Matrix metalloproteinase-3, MMP-3NCBI Gene Id:403445Protein Name:stromelysin-1Swissprot Id:Q6Y4Q5Protein Accession #:NP_001002967.1Nucleotide Accession #:NM_001002967.1Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express MMP3. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express MMP3. Aviva为每个蛋白质表达项目提供定制的解决方案。我们专业的科研人员对困难的蛋白质具有丰富的经验，并已生产出2500多种重组蛋白质。无论您的需求是小型研究还是大型cGMP，我们都可以运用我们的经验来满足您的需求。服务包括大肠杆菌表达，哺乳动物表达，酵母表达和抗原制备。
