Predicted Species Reactivity:BovineApplication:ELISAKit Detection Method:Colorimetric, OD450 nmKit Principle:Aviva Systems Biology MMP2 ELISA Kit (Bovine) (OKEH07289) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MMP2 captured in well.Kit Range:0.312 - 20ng/mLKit Recovery:Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90% (n = 20)Kit Reproducibility:Mean Intra-assay CV%: <=7.2% (n="">Mean Inter-assay CV%: <=9.2% (n="">Kit Component:| Component | Amount |
|---|
| MMP2 Microplate | 96 Wells (12 x 8 Well strips) |
| MMP2 Lyophilized Standard | 2 |
| 100X Biotinylated MMP2 Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Sample Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| TMB Substrate | 1 x 10 mL |
| Stop Solution | 1 x 10 mL |
Additional Information:Function: PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrin alpha-v/beta-3 on the surface of blood vessels.:::Subcellular Location: Secreted Extracellular space Extracellular matrix Membrane Nucleus Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes (By similarity).:::Unigene: Bt.5313SMR: Q9GLE5String: 9913.ENSBTAP00000025657:::KEGG: bta:282872Pfam: PF00040:::KEGG: bta:282872Pfam: PF00040Reconstitution and Storage:Store as indicated in product manual.Sensitivity:0.15ng/mLDatasheets/Manuals:Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.Specificity:Natural and recombinant Bovine 72 kDa type IV collagenaseAssay Info:Assay Methodology: Quantitative Sandwich ELISAGene Symbol:MMP2Official Gene Full Name:matrix metallopeptidase 2Alias Symbols:72 kDa gelatinase, Matrix metalloproteinase-2, MMP-2NCBI Gene Id:282872Protein Name:72 kDa type IV collagenaseSwissprot Id:Q9GLE5Protein Accession #:NP_777170.1Nucleotide Accession #:NM_174745.2Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express MMP2. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express MMP2. Aviva为每个蛋白质表达项目提供定制的解决方案。我们专业的科研人员对困难的蛋白质具有丰富的经验，并已生产出2500多种重组蛋白质。无论您的需求是小型研究还是大型cGMP，我们都可以运用我们的经验来满足您的需求。服务包括大肠杆菌表达，哺乳动物表达，酵母表达和抗原制备。
