Predicted Species Reactivity:HumanProduct Format:Purified IgG - lyophilised Clonality:PolyclonalIsotype:Polyclonal IgGHost:RabbitApplication:WB:::Preservative Stabilisers: None presentAntiserum Preparation: Antisera to human MCP-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.:::Approx Protein Conc: IgG concentration 1.0 mg/mlBuffer Solutions: Phosphate buffered saline pH7.4Reconstitution and Storage:Reconstitution: Reconstitute with 0.1 ml distilled water.For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.Storage: Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Replacement Item:This antibody may replace item sc-130328 from Santa Cruz Biotechnology.Immunogen:The immunogen for anti-CCL2 antibody: recombinant human MCP-1Predicted Homology Based on Immunogen Sequence:HumanDatasheets/Manuals:Printable datasheet for anti-CCL2 antibody - OASA07962Specificity:MCP-1Application Info:ELISA: 0.5ug/mlWestern Blotting: 0.1ug/ml - 0.2ug/mlFunctional Assays: 2.0ug/ml - 3.0ug/ml following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.| Yes | No | Not Determined | Suggested Dilution | | | |  | |
| | | | |
| | | | |
| | | | |
| | | 0.5ug/ml |
| | | | |
| | | 0.1ug/ml - 0.2ug/ml |
| | | 2.0ug/ml - 3.0ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using apropriate negative/positive controls.
Target Reference:1. Banisor, I. et al. (2005) Involvement of beta-chemokines in the development of inflammatory demyelination. J. Neuroinflammation. 2: 7-20.Gene Symbol:CCL2Alias Symbols:HC11, MCAF, MCP1, MCP-1, SCYA2, GDCF-2, SMC-CF, HSMCR30, MGC9434, CCL2NCBI Gene Id:6347Protein Name:C-C motif chemokine 2Description of Target:RABBIT ANTI HUMAN MCP-1Swissprot Id:P13500Protein Accession #:NP_002973.1Protein Size (# AA):99Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express CCL2. 
RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express CCL2. 
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