Predicted Species Reactivity:Human, Mouse, RatClonality:MonoclonalClone:S24-18Isotype:IgG1Host:MouseApplication:WB, IHC, IPAdditional Information:Background Info: Detects ~300kDa. Does not recognize type 2 or type 3 IP3R"s.:::Scientific Background: Inositol 1,4,5-triphosphate receptor, also known as IP3R or InsP3R, is a member of the intracellular calcium releasechannel family, and is located in the endoplasmic reticulum.It functions as a Ca2+ release channel for intracellular storesof calcium ions. There are three types of IP3 receptors (IP3receptor 1, 2 and 3) that require the second messengerinositol 1,4,5-triphosphate (IP3) for activation (3). Fourindividual receptor subunits, resulting in homo- or hetero-oligomerizationof the receptor isoform, form a functionalchannel (4). Phosphorylation of IP3R1 at Ser1756 by cyclic-AMP-dependent protein kinase A (PKA) regulates thesensitivity of IP3R1 to IP3 and may thus be a mode ofregulation for Ca2+ release (5, 6). IP3R1 mediated calciumrelease appears to have an effect on the induction of longterm depression (LTD) in Purkinje cells (7).:::Certificate of Analysis: 1ug/mL was sufficient for detection of IP3 in 20ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.Reconstitution and Storage:Store at -20C. Shipping in Blue Ice or 4C.Replacement Item:This antibody may replace item HPA016487Immunogen:Fusion protein amino acids 2680-2749 (cytoplasmic carboxyl terminus) of rat type1 IP3R inositol 1,4,5-triphosphate receptor type 1, Itpr1Purification:Protein G PurifiedConcentration:1mg/mLDatasheets/Manuals:Printable datasheet for OASE00236Dilution:1ug/mL (WB), 0.1-1ug/mL (IHC/ICC), 1-10ug/mL (IF)Storage Buffer:PBS pH7.4, 50% glycerolTarget Reference:1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rdEd., Sinauer Associated Inc.: Sunderland, MA USA.2. www.iochannels.org3. Joseph S.K. (1996) Cell Signal 8: 1-7.4. Galvan D.L., et al. (1999) J Biol Chem 274: 29483-29492.5. Haug L.S., et al. (1999) J Biol Chem 274: 7467-7473.6. DeSouza N., et al. (2002) J Biol Chem 277: 39397-39400. 7. Inoue T., et al. (1998) J Neurosci 18: 5366-5373.Gene Symbol:Itpr1Official Gene Full Name:inositol 1,4,5-trisphosphate receptor, type 1NCBI Gene Id:25262Swissprot Id:P29994Protein Accession #:NP_001007236.1Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express Itpr1. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express Itpr1. Aviva为每个蛋白质表达项目提供定制的解决方案。我们专业的科研人员对困难的蛋白质具有丰富的经验，并已生产出2500多种重组蛋白质。无论您的需求是小型研究还是大型cGMP，我们都可以运用我们的经验来满足您的需求。服务包括大肠杆菌表达，哺乳动物表达，酵母表达和抗原制备。
