Catalog Number:  26052

Gene Symbol:  EYA2

Description:   Anti- EYA2 Mouse Monoclonal Antibody

Background:   EYA2 (eyes absent homolog 2)functions as a tyrosine phosphatase that specificallydephosphorylates Tyr-142 of histone H2AX. EYA2 promotes efficientDNA repair and coactivates SIX1. Together with SIX1 and DACH2, EYA2seems to be involved in myogenesis.

Immunogen:   A synthetic peptide near theN-terminus of human EYA2.

Applications:   ELISA, WB, IHC, IP

Recommended Dilutions:    ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:100

Concentration:   1 mg/ml

Host:   mouse

Format:   Liquid

Clonality:   Monoclonal

Isotype:   IgG

Purity:   Purified from ascites

Storage buffer:

Preservative:  no

Constituents:  PBS (without Mg2+and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity:  Anti-EYA2 antibody recognizesEYA2 of vertebrates.

Storage Conditions:  Store at -20°C. Avoid freeze /thaw cycles.

Download Datasheet:

Western blot:

	Western blot analysis of recombinant full-length EYA2 protein.Purified His-tagged EYA2 protein (Cat. # 10195) was blotted withanti-EYA2 monoclonal antibody (Cat. # 26052).
Immunohistochemistry:

Immunohistochemical analysis of paraffin-embedded humanadenocarcinoma tissue with anti-EYA2 monoclonal antibody (Cat. #26052). Tissue section was fixed with formaldehyde and blocked with1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediationin citrate buffer (pH6). Samples were then incubated with primaryantibody (1:50) overnight at 4°C. A HRP-conjugated Goat anti-mouseIgG (dilution 1:50) was used as secondary antibody.

Related Products
Catalog#	Name	Size	Price	DataSheet
26052	Anti- EYA2 Mouse Monoclonal Antibody	100 μL	$239
26096	Anti- EYA2 Mouse Monoclonal Antibody	100 μL	$239
21128	Anti- EYA2 Rabbit Polyclonal Antibody	100 μL	$219

定制肽合成NewEast Biosciences为全球生物研究界提供高质量的粗制和纯化肽或拟肽。我们使用先进的自动，半自动和手动合成器以及Fmoc技术合成肽。肽酰胺键通过HOAT / HATU偶联形成。我们的肽通过制备型HPLC纯化，并且每个级分均通过使用不同溶剂系统的分析型HPLC进行进一步分析。收集并冻干仅通过质谱法验证的具有单个峰的级分。肽通常在1-2周内递送。粗肽也可以大量节省（参见价格表）。周转时间为5-7天。除了正常的肽合成外，我们还合成修饰的肽和多种抗原性肽（MAPs）。修饰在C末端，N末端和内部残基处进行。在C末端，氨基可与羧酸，异氰酸酯和磺酰氯反应形成酰胺，脲和磺酰胺。在此终端进行经典的生物素化，荧光标记和脂质化。这些修饰以及磷酸化和糖基化也可以在内部残基处进行。相反，在C末端的修饰非常具有挑战性，需要溶液阶段的干预。为了在C端进行修饰，必须将肽从树脂中释放出来。然后，用相应的化学方法修饰释放的肽。但是，我们已经开发了适当的化学方法，可以在固相的C末端引入生物素，荧光染料和胺。随着化学，我们降低了合成肽以及昂贵试剂的成本。作为NewEast的客户，我们将节省下来的钱转嫁给您。