NewEast/Fascin(内肽)[26013][100µL]/26013

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¥4780.00
货号:26013
浏览量:96
品牌:NewEastBio
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商品描述

Catalog Number:  26013
Gene Symbol:  FSCN1
Description:   Anti-Fascin Mouse Monoclonal Antibody
Background:   Fascin is an ~58 kDa monomeric actin filament bundling protein. It is required to maximally cross-link the actin filaments into straight, compact, and rigid bundles, and contributes to the formation of filopodia that are critical for cell migration. Elevated levels of fascin have been found in metastatic tumors and are correlated with clinically aggressive phenotypes, poor prognosis, and shorter survival.
Immunogen:   A peptide from the middle of human Fascin 1
Applications:   ELISA, WB, IP, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:100-1:2000
IP: 1 µ for 1~2 mg total cellular proteins
IHC: 1:100 dilution
Concentration:   1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-fascin antibody recognizes fascin 1, 2 and 3 of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
Download Datasheet:  
Immunofluorescence:  
Detection of fascin by immunofluorescence in 4T1 mouse breast tumor cells with antifascin monoclonal antibody (Cat # 26013).
 
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定制肽合成NewEast Biosciences为全球生物研究界提供高质量的粗制和纯化肽或拟肽。我们使用先进的自动,半自动和手动合成器以及Fmoc技术合成肽。肽酰胺键通过HOAT / HATU偶联形成。我们的肽通过制备型HPLC纯化,并且每个级分均通过使用不同溶剂系统的分析型HPLC进行进一步分析。收集并冻干仅通过质谱法验证的具有单个峰的级分。肽通常在1-2周内递送。粗肽也可以大量节省(参见价格表)。周转时间为5-7天。除了正常的肽合成外,我们还合成修饰的肽和多种抗原性肽(MAPs)。修饰在C末端,N末端和内部残基处进行。在C末端,氨基可与羧酸,异氰酸酯和磺酰氯反应形成酰胺,脲和磺酰胺。在此终端进行经典的生物素化,荧光标记和脂质化。这些修饰以及磷酸化和糖基化也可以在内部残基处进行。相反,在C末端的修饰非常具有挑战性,需要溶液阶段的干预。为了在C端进行修饰,必须将肽从树脂中释放出来。然后,用相应的化学方法修饰释放的肽。但是,我们已经开发了适当的化学方法,可以在固相的C末端引入生物素,荧光染料和胺。随着化学,我们降低了合成肽以及昂贵试剂的成本。作为NewEast的客户,我们将节省下来的钱转嫁给您。