CatalogNumber:  21001

GeneSymbol:  Orai1

Description:  Anti-Orai1RabbitPolyclonalAntibody(Orai1-L1)

Background:  Store-operatedcalciumchannels(SOCs)playessentialfunctionsinvariousphysiologicalprocesses.Orai1(alsonamedCRACM1)isacriticalmolecularcomponentofSOCs.Orai1proteinisa~35kDaplasmamembraneproteinwith4transmembranedomains.Humanpatientswithaseverecombinaedimmunodeficiency(SCID)syndromepossesspointmutationsinOrai1gene.

Immunogen:  Apeptidefromtheextracellularloop1regionofhumanOrai1

Applications:  ELISA,WB,IHC

RecommendedDilutions:  ELISA:      1:1000-1:5000,WB:          1:1000-1:2000,IHC:          1:100-1:200

Concentration:

Host:  Rabbit

Format:  Liquid

Clonality:  Polyclonal

Isotype:  IgG

Purity:  Immunogenaffinitypurified

Storagebuffer:

Preservative:  0.01%sodiumazide

Constituents:  50mMNaHCO3-Glycine,0.15MNaCl,pH7.4

SpeciesReactivity:  Orai1-L1antibodyrecognizesOrai1fromhumanandmouse.ItmayalsorecognizeOrai2andOrai3.

StorageConditions:  Storeat-20°C.Avoidfreeze/thawcycles

DownloadDatasheet:

Westernblot:

	WesternblotanalysisofOrai1protein.HumanOrai1plasmidDNAwastransientlytransfectedintoHEK-293cells.Whole-celllysatewasusedforwesternblotintheabsence(lane1)orpresence(lane2)ofantigenpeptide.
Immunohistochemistry
	ImmunohistochemistryofendogenousOrai1inNIH3T3cellsshowingtheplasmamembranestaining.

RelatedProducts
Catalog#	Name	Size	Price	DataSheet
21001	Anti-Orai1RabbitPolyclonalAntibody(Orai1-L1)	100μL	$219
21002	Anti-Orai1RabbitPolyclonalAntibody(Orai1-L2)	100μL	$219

定制肽合成NewEast Biosciences为全球生物研究界提供高质量的粗制和纯化肽或拟肽。我们使用先进的自动，半自动和手动合成器以及Fmoc技术合成肽。肽酰胺键通过HOAT / HATU偶联形成。我们的肽通过制备型HPLC纯化，并且每个级分均通过使用不同溶剂系统的分析型HPLC进行进一步分析。收集并冻干仅通过质谱法验证的具有单个峰的级分。肽通常在1-2周内递送。粗肽也可以大量节省（参见价格表）。周转时间为5-7天。除了正常的肽合成外，我们还合成修饰的肽和多种抗原性肽（MAPs）。修饰在C末端，N末端和内部残基处进行。在C末端，氨基可与羧酸，异氰酸酯和磺酰氯反应形成酰胺，脲和磺酰胺。在此终端进行经典的生物素化，荧光标记和脂质化。这些修饰以及磷酸化和糖基化也可以在内部残基处进行。相反，在C末端的修饰非常具有挑战性，需要溶液阶段的干预。为了在C端进行修饰，必须将肽从树脂中释放出来。然后，用相应的化学方法修饰释放的肽。但是，我们已经开发了适当的化学方法，可以在固相的C末端引入生物素，荧光染料和胺。随着化学，我们降低了合成肽以及昂贵试剂的成本。作为NewEast的客户，我们将节省下来的钱转嫁给您。

Publications:

1.  GenerationandCharacterizationofFullyHumanMonoclonalAntibodiesAgainstHumanOrai1forAutoimmuneDisease    JPharmacolExpTher.2013May;345(2):225-38

2.  InfluenceofOrai1interventiononmouseairwayepitheliumreactionsinvivoandinvitro    AnnAllergyAsthmaImmunol.2012Feb;108(2):103-12

3.  2-AminoethoxydiphenylborateadmiNISTrationintothenostrilalleviatesmurineallergicrhinitis    AmJOtolaryngol.2011Jul-Aug;32(4):318-28

4.  Up-regulationofOrai1inmurineallergicrhinitis    HistochemCellBiol.2010Jul;134(1):93-102

5.  Orai1andSTIM1arecriticalforbreasttumorcellmigrationandmetastasis    CancerCell.2009Feb3;15(2):124-34

6.  AmeliorationofMuc5bmucinhypersecretionisenhancedbyIL-33after2-APBadministrationinamurinemodelofallergicrhinitis    BiotechHistochem.2014May;89(4):273-86