ProductHighlight- BasedontheuniquemousemonoclonalAnti-cGMPantibodyItistheonlyEIAkitonthemarketwithmonoclonalanti-cGMPantibody(allotherkitsarebasedonpolyclonalanti-cGMPantibodies).
- Nomorehazardouschemical(aceticanhydride)foryourcGMPassayThemonoclonalAnti-cGMPantibodyhassimilaraffinitiesfornon-acetylatedcGMPandacetylatedcGMP.Thusacetylationwiththeaceticanhydrideispermanentlyeliminatedfromtheassay.
- SupersensitivityandselectivityThemonoclonalAnti-cGMPantibodydisplays>108foldofselectivityovercAMP,GTP,andothernucleosideanalogues.TheAnti-cGMPEIAKitprovidessignificantlyimprovedsensitivityandselectivityoverotherkitsbasedonpolyclonalanti-cGMPantibodiesonthemarket.
- Shortestassaytimeandhigh-throughputformatTheAnti-cGMPEIAKitisthebestchoicefordrugscreenings.
ProductDescriptionGuanosine3",5"-cyclicmonophosphate(cyclicGMP;cGMP)playscriticalregulatoryrolesinmanyphysiologicalprocesses.cGMPisproducedfromGTPbyguanylylcyclasesandisdegradedbyphosphodiesterases.StimulationofguanylylcyclasesorinhibitionofphosphodiesterasescanincreasecellularcGMPconcentrations.InhibitorsofthecGMP-specificphosphodiesterasesareusedfortreatinghumandiseases.Forexample,inhibitorsofcGMPspecificphosphodiesterasetype5(suchasViagraandCialis)areusedfortreatingerectiledysfunction. Toscreenforinhibitorsofphosphodiesterasesorstimulatorsofguanylylcyclases,itisessentialtohaveafast,sensitive,selectiveandreproducIBLemethodtomeasurethecGMPconcentrations.Thisisespeciallytruefortheinitialscreeningsgiventhepossibleweakereffectsoflargerpoolsofcompounds. CurrentlyavailableotherELISAkitsmeasuringcGMPlevelsarebasedonthenon-affinity-purifiedpolyclonalanti-cGMPantibody.Despitetheclaimedselectivity,thesepolyclonalanti-cGMPantibodiesdisplaycertaincross-reactivitywithcAMPorGTP.Inmostcelltypes,cGMPispresentatlevels~100foldlowerthancAMP. NewEastBiosciencescGMPELISAkitisbasedontheuniquemousemonoclonalanti-cGMPantibody.Thismonoclonalanti-cGMPantibodydisplays>108foldofselectivityovercAMP,GTP,andothernucleosideanalogues.NewEastBiosciencescGMPELISAkitprovidessignificantlyimprovedsensitivityandselectivityoverotherkitsbasedonpolyclonalanti-cGMPantibodies.Ourmonoclonalanti-cGMPantibody-basedELISAkitalsoavoidsthebatch-to-batchvariationsassociatedwithpolyclonalantibodyproductionsfromanimals,thusprovidingthereproducibilityinthelongrun. FurThermore,whilepolyclonalanti-cGMPantibodiesusedinotherELISAkitshavehigheraffinityforacetylatedcGMPthannon-acetylatedcGMP,NewEastBiosciencesmonoclonalanti-cGMPantibodyhassimilaraffinitiestonon-acetylatedandacetylatedcGMPmolecules.Therefore,acetylationtreatmentsofsamplesandstandardsarenotneededinNewEastBiosciencescGMPELISAkit.Thissignificantlyreducesthetimefortheassay.Theavoidanceoforganicreagentsusedintheacetylationprocessprovidesasafeandhealthyworkenvironment. PrincipleOutlineNewEastBiosciencescGMPELISAKitisacompetitiveimmunoassaytomeasurethecGMPlevels,eitherfromcellextractsorfrominvitroguanylylcyclaseassays.Briefly,multi-wellplatesarecoatedwithgoat-anti-mouseserum.cGMPincellextractsorininvitroguanylylcyclaseassayswillcompetitivelybindtothemonoclonalanti-cGMPantibodyinthepresenceoffixedamountsofcGMP-conjugatedhorse-rADIshperoxidase.KnownamountsofcGMPareusedasstandardstogeneratethecalculationcurve.Afterashortincubation,theexcessreagentsarewashedawayandsubstrateisadded.Themultiwellplatesarethenreadonamicroplatereaderat450nm.TheintensityoftheyellowcolorisinverselyproportionaltotheconcentrationofcGMPinsamples.ThemeasuredopticaldensityisusedtocalculatetheconcentrationofcGMPinsamplesbasedonthecurvefromthecGMPstandards.  FAQs
ProductManual AssayLayoutSheet Publications:| 1. Sulfhydryl-dependentdimerizationofsolubleguanylylcyclasemodulatestherelaxationofporcinepulmonaryarteriestonitricoxide PflugersArch.2013Feb;465(2):333-41 | | 2. Pressure-overload-inducedsubcellularrelocalization/oxidationofsolubleguanylylcyclaseintheheartmodulatesenzymestimulation CircRes.2012Jan20;110(2):295-303 | | 3. Natriureticpeptidesblocksynaptictransmissionbyactivatingphosphodiesterase2AandreducingpresynapticPKAactivity ProcNatlAcadSciUSA.2012Oct23;109(43):17681-6 | | 4. Roleofsulfhydryl-dependentdimerizationofsolubleguanylylcyclaseinrelaxationofporcinecoronaryarterytonitricoxide CardiovascRes.2011Jun1;90(3):565-72 | | 5. Molt-inhibitinghormonefromChinesemittencrab(Eriocheirsinensis):Cloning,tissueexpressionandeffectsofrecombinantpeptideonecdysteroidsecretionofYOs GenCompEndocrinol.2011Sep15;173(3):467-74 | | 6. InhibitionofStriatalSolubleGuanylylCyclase-cGMPSignalingReversesBasalGangliaDysfunctionandAkinesiainExperimentalParkinsonism PLoSOne.2011;6(11):e27187 | | 7. Molecularanalysis,developmentalfunctionandheavymetal-inducedexpressionofABCC5inzebrafish CompBiochemPhysiolBBiochemMolBiol.2011Jan;158(1):46-55 | | 8. FeedbackcontrolthroughcGMP-dependentproteinkinasecontributestodifferentialregulationandcompartmentationofcGMPinratcardiacmyocytes CircRes.2010Nov12;107(10):1232-40 | | 9. TheSolubleGuanylateCyclaseActivatorBAY58-2667ProtectsagainstMorbidityandMortalityinEndotoxicShockbyRecouplingOrganSystems PLoSOne.2013Aug28;8(8):e72155 | | 10. Stimulationofguanylylcyclase-Dbybicarbonate Biochemistry.2009May26;48(20):4417-22 | | 11. Volumeoverloadinducesdifferentialspatiotemporalregulationofmyocardialsolubleguanylylcyclaseineccentrichypertrophyandheartfailure JournalofMolecularandCellularCardiologyVolume60,July2013,Pages72–83 | | 12. DefiningSpecificityDeterminantsofCyclicGMP-MediatedGustatorySensoryTransductioninCaenorhaBDitiselegans genetics.113.152660v1194/4/885 | | 13. Theroleofnitricoxidesignalinginfoodintake;insightsfromtheinnermitochondrialmembranepeptidase2mutantmice RedoxBIOLOGyVolume1,Issue1,2013,Pages498–507 | | 14. CrossregulationbetweencGMP-dependentproteinkinaseandAktinvasodilatationofporcinepulmonaryartery JournalofCardiovascularPharmacology:November2014-Volume64-Issue5-p452-459 | | 15. Geneticablationofsolutecarrierfamily7a3aleadstohepaticsteatosisinzebrafishduringfasting HepatologyVolume60,Issue6,pages1929–1941,December2014 | 定制肽合成NewEast Biosciences为全球生物研究界提供高质量的粗制和纯化肽或拟肽。我们使用先进的自动，半自动和手动合成器以及Fmoc技术合成肽。肽酰胺键通过HOAT / HATU偶联形成。我们的肽通过制备型HPLC纯化，并且每个级分均通过使用不同溶剂系统的分析型HPLC进行进一步分析。收集并冻干仅通过质谱法验证的具有单个峰的级分。肽通常在1-2周内递送。粗肽也可以大量节省（参见价格表）。周转时间为5-7天。除了正常的肽合成外，我们还合成修饰的肽和多种抗原性肽（MAPs）。修饰在C末端，N末端和内部残基处进行。在C末端，氨基可与羧酸，异氰酸酯和磺酰氯反应形成酰胺，脲和磺酰胺。在此终端进行经典的生物素化，荧光标记和脂质化。这些修饰以及磷酸化和糖基化也可以在内部残基处进行。相反，在C末端的修饰非常具有挑战性，需要溶液阶段的干预。为了在C端进行修饰，必须将肽从树脂中释放出来。然后，用相应的化学方法修饰释放的肽。但是，我们已经开发了适当的化学方法，可以在固相的C末端引入生物素，荧光染料和胺。随着化学，我们降低了合成肽以及昂贵试剂的成本。作为NewEast的客户，我们将节省下来的钱转嫁给您。
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