CatalogNumber:  10138

ProductName:  Gα13Protein

Synonyms:  Guaninenucleotide-bindingproteinsubunitalpha-13,Galpha13,G13

Source:  Human,recombinantfulllength,His6-tag

ExpressionHost:  sf9cells

MolecularWeight:  44kDa

Purity:  >95%bySDS-PAGE

Introduction:  Ga13belongstotheG12familyofheterotrimericguaninenucleotide-bindingproteins,andhasintrinsicGTPaseactivity.Ga13hasbeenrevealedtoplaycriticalrolesintransformation,normalhemostasisandthrombosis,growthfactor-inducedcellmigration,angiogenesis,andsalt-inducedhypertension.

AminoAcidSequence  (1-377)

MADFLPSRSVLSVCFPGCLLTSGEAEQQRKSKEIDKCLSREKTYVKRLVKILLLGAGESGKSTFLKQ MRIIHGQDFDQRAREEFRPTIYSNVIKGMRVLVDAREKLHIPWGDNSNQQHGDKMMSFDTRAPMAAQ GMVETRVFLQYLPAIRALWADSGIQNAYDRRREFQLGESVKYFLDNLDKLGEPDYIPSQQDILLARRPTK GIHEYDFEIKNVPFKMVDVGGQRSERKRWFECFDSVTSILFLVSSSEFDQVLMEDRLTNRLTESLNIFETIVNNRVFSNVSIILFLNKTDLLEEKVQIVSIKDYFLEFEGDPHCLRDVQKFLVECFRNKRRDQQQKPLYHHFTTAINTENIRLVFRDVKDTILHDNLKQLMLQ

Properties

PhysicalAppearance(form):  Dissolvedin20mMTris-HCl,pH8.0,150mMNaCl.

PhysicalAppearance(form):  Whiteorclear

Concentration: 1mg/mL

Storage:  -80°C

PreparationInstructions:  Centrifugethevialbeforeopenthecapandreconstituteinwater.Addingof10mMβ-mercaptoethanolor1mMDTTintothesolutiontoprotecttheproteinisrecommendedandusingofnon-ionicdetergentssuchasn-Dodecylβ-D-maltoside(DoDM)orpolyethylenedetergents(e.g.C12E10)alsohelptostABIlizetheprotein.Avoidrepeatedfreezingandthawingafterreconstitution.ThepurityofHis-taggedGα13wasdeterminedbySDS-PAGEandCoomassieBrilliantBlueStaining.

References:  1.Gong,H.etal.,Science327:340-343,2010.2.Kabouridis,P.S.etal.,Molec.Cell.Biochem.144:45-51,1995.3.Kilts,J.D.etal.,J.Cardiovasc.Pharm.50:299-303,2007.4.Moers,A.etal.,NatureMed.9:1418-1422,2003.5.Offermanns,S.etal,Science275:533-536,1997.6.Radhika,V.etal.,J.Biol.Chem.279:49406-49413,2004.7.Ruppel,K.M.etal.,Proc.Nat.Acad.Sci.102:8281-8286,2005.8.Shan,D.etal.,Dev.Cell10:707-718,2006.9.Wirth,A.etal.,NatureMed.14:64-68,2008.

Datasheet:

定制肽合成NewEast Biosciences为全球生物研究界提供高质量的粗制和纯化肽或拟肽。我们使用先进的自动，半自动和手动合成器以及Fmoc技术合成肽。肽酰胺键通过HOAT / HATU偶联形成。我们的肽通过制备型HPLC纯化，并且每个级分均通过使用不同溶剂系统的分析型HPLC进行进一步分析。收集并冻干仅通过质谱法验证的具有单个峰的级分。肽通常在1-2周内递送。粗肽也可以大量节省（参见价格表）。周转时间为5-7天。除了正常的肽合成外，我们还合成修饰的肽和多种抗原性肽（MAPs）。修饰在C末端，N末端和内部残基处进行。在C末端，氨基可与羧酸，异氰酸酯和磺酰氯反应形成酰胺，脲和磺酰胺。在此终端进行经典的生物素化，荧光标记和脂质化。这些修饰以及磷酸化和糖基化也可以在内部残基处进行。相反，在C末端的修饰非常具有挑战性，需要溶液阶段的干预。为了在C端进行修饰，必须将肽从树脂中释放出来。然后，用相应的化学方法修饰释放的肽。但是，我们已经开发了适当的化学方法，可以在固相的C末端引入生物素，荧光染料和胺。随着化学，我们降低了合成肽以及昂贵试剂的成本。作为NewEast的客户，我们将节省下来的钱转嫁给您。

Publications:

1.  Resistancetoinhibitorsofcholinesterase-8A(Ric-8A)iscriticalforgrowthfactorreceptor-inducedactincytoskeletalreorganization    JBiolChem.2011Sep2;286(35):31055-61

2.  AgoNISTactivationoftheGprotein-coupledreceptorGPR35involvestransmembranedomainIIIandistransducedviaGalphaandbeta-arrestin-2    BrJPharmacol.2011Feb;162(3):733-48

3.  Gα12/13inhibitionenhancestheanti-cancereffectofbortezomibthroughPSMB5downregulation    Carcinogenesis(2010)

4.  SuppressionoftumorangiogenesisbyGalpha(13)haploinsufficiency    JBiolChem.2009Oct2;284(40):27409-15