CatalogNumber:  10107

ProductName:  Cdc42Protein

Synonyms:  Celldivisioncycle42,G25K,CDC42Hs

Source:  Human,recombinantfulllength,His6-tag

ExpressionHost:  E.coli

MolecularWeight:  21kDa

Purity:  >95%bySDS-PAGE

Introduction:  SmallGTPasesareasuper-familyofcellularsignalingregulators.Cdc42belongstotheRhosub-familyofGTPasesthatregulatecellmotility,celldivision,andgenetranscription.GTPbindingincreasestheactivityofCdc42,andthehydrolysisofGTPtoGDPrendersitinactive.GTPhydrolysisisaidedbyGTPaseactivatingproteins(GAPs),whileexchangeofGDPforGTPisfacilitatedbyguaninenucleotideexchangefactors(GEFs).

AminoAcidSequence  (1-191)

MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRL RPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQ KPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALEPPEPKKSRRCVLL

Properties

PhysicalAppearance(form):  Dissolvedin20mMTris-HCl,pH8.0,150mMNaCl.

PhysicalAppearance(form):  Whiteorclear

Concentration:  1mg/mL

Storage:  -80°C

PreparationInstructions:  Centrifugethevialbeforeopenthecapandreconstituteinwater.Addingof10mMβ-mercaptoethanolor1mMDTTintothesolutiontoprotecttheproteinisrecommendedandusingofnon-ionicdetergentssuchasn-Dodecylβ-D-maltoside(DoDM)orpolyethylenedetergents(e.g.C12E10)alsohelptostABIlizetheprotein.Avoidrepeatedfreezingandthawingafterreconstitution.ThepurityofHis-taggedCdc42wasdeterminedbySDS-PAGEandCoomassieBrilliantBlueStaining.

References:  1.Garrett,W.S..etal.,Cell102:325-334,2000.2.Irie,F.etal.,NatureNeurosci.5:1117-1118,2002.3.Kawasaki,Y.etal.,Oncogene26:7620-7627,2007.4.Manser,E.etal.,Nature363:364-367,1993.5.Musch,A.etal.,EMBOJ.20:2171-2179,2001.6.Nalbant,P.etal.,Science305:1615-1619,2004.7.Shen,Y.etal.,Dev.Cell14:342-353,2008.8.Wu,W.J.etal.,Nature405:800-804,2000.9.Wu,X.etal.,GenesDev.20:571-585,2006.10.Zheng,Y.etal.,J.Biol.Chem.271:33169-33172,1996.

Datasheet:

定制肽合成NewEast Biosciences为全球生物研究界提供高质量的粗制和纯化肽或拟肽。我们使用先进的自动，半自动和手动合成器以及Fmoc技术合成肽。肽酰胺键通过HOAT / HATU偶联形成。我们的肽通过制备型HPLC纯化，并且每个级分均通过使用不同溶剂系统的分析型HPLC进行进一步分析。收集并冻干仅通过质谱法验证的具有单个峰的级分。肽通常在1-2周内递送。粗肽也可以大量节省（参见价格表）。周转时间为5-7天。除了正常的肽合成外，我们还合成修饰的肽和多种抗原性肽（MAPs）。修饰在C末端，N末端和内部残基处进行。在C末端，氨基可与羧酸，异氰酸酯和磺酰氯反应形成酰胺，脲和磺酰胺。在此终端进行经典的生物素化，荧光标记和脂质化。这些修饰以及磷酸化和糖基化也可以在内部残基处进行。相反，在C末端的修饰非常具有挑战性，需要溶液阶段的干预。为了在C端进行修饰，必须将肽从树脂中释放出来。然后，用相应的化学方法修饰释放的肽。但是，我们已经开发了适当的化学方法，可以在固相的C末端引入生物素，荧光染料和胺。随着化学，我们降低了合成肽以及昂贵试剂的成本。作为NewEast的客户，我们将节省下来的钱转嫁给您。

Publications:

1.  VibrioparahaemolyticusEffectorProteinsSuppressInflammasomeActivationbyInterferingwithHostAutophagySignaling    PLoSPathog.2013Jan;9(1):e1003142

2.  Interferongammainducesactinpolymerization,Rac1activationanddownregulatesphagocytosisinhumanmonocyticcells    Cytokine.2012Jan;57(1):158-68

3.  Invadopodiaandrolling-typemotilityarespecificfeaturesofhighlyinvasivep190bcr-ablleukemiccells    EuropeanJournalofCellBIOLOGyVolume91,Issues11–12,November–December2012,Pages978–987

4.  EpithelialjunctionformationrequiresconfinementofCdc42activitybyanovelSH3BP1complex    JCellBiol.2012Aug20;198(4):677-93

5.  PolarizedmigrationoflymphaticendothelialcellsiscriticallydependentonpodoplaninregulationofCdc42    AmJPhysiolLungCellMolPhysiol.2011Jan;300(1):L32-42

6.  TheAarskog-ScottSyndromeProteinFgd1RegulatesPodosomeFormationandExtracellularMatrixRemodelinginTransformingGrowthFactorbeta-StimulatedAorticEndothelialCells    MolCellBiol.2011Nov;31(22):4430-41

7.  MMP-9andCXCL8/IL-8ArePotentialTherapeuticTargetsinEpidermolysisBullosaSimplex    PLoSOne.2013Jul19;8(7):e70123

8.  p21-ActivatedKinase(PAK)RegulatesCytoskeletalReorganizationandDirectionalMigrationinHumanNeutrophils    PLoSOne.2013Sep3;8(9):e73063

9.  TheCdc42GuanineNucleotideExchangeFactorFGD6CoordinatesCellPolarityandEndosomalMembraneRecyclinginOsteoclasts    JBiolChem.2014Jun27;289(26):18347-59

10.  Cdc42InhibitorandUsesThereof    UnitedStatesPatentApplication20140194451

11.  Theα4NicotinicReceptorPromotesCD4+T-CellProliferationandaHelperT-CellImmuneResponse    MolecularPharmacologyJanuary2014vol.85no.150-61