CatalogNumber:  26253

GeneSymbol:  FGFR1,BFGFR,CD331,CEK,FGFBR,FLG

Description:  Anti-FGFR1(P252T)MouseMonoclonalAntibody

Background:  TheproteinFGFR1ishighlyconservedbetweenmembersandthroughoutevolution.ThisparticularfamilymemberofFGFR1bindsbothacidicandbasicfibroblastgrowthfactorsandisinvolvedinlimbinduction.MutationsofFGFR1proteinhavebeenassociatedwithPfeiffersyndrome,Jackson-Weisssyndrome,Antley-Bixlersyndrome,osteoglophonicdysplasia,squamouscelllungcancerandautosomaldominantKallmannsyndrome.

Immunogen:  AsyntheticpeptidefromtheinternalregionofFGFR1whichincludesthemutationofP252T,humanorigin.

Applications:  ELISA,WB,IHC

RecommendedDilutions:  ELISA:1:1000-1:2000WB:1:500-1:1000IHC:1:50-1:100

Concentration:  1mg/ml

Host:  Mouse

Format:  Liquid

Clonality:  Monoclonal

Isotype:

Purity:  Purifiedfromascites

Storagebuffer:

Preservative:  no

Constituents:  PBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,50%glycerol

SpeciesReactivity:  RecognizesP252Tmutant,butnotwild-typeFGFR1ofvertebrates.

StorageConditions:  Storeat-20°C.Avoidfreeze/thawcycles

DownloadDatasheet:

Westernblot:

WesternblotanalysisofrecombinantFGFR1(P252T)andwildtypeproteins.PurifiedHis-taggedFGFR1(P252T)protein(22-376aa,~45KDa,lane2)andcorrespondingwildtypeprotein(lane1)wereblottedwithanti-FGFR1(P252T)monoclonalantibody(Cat.#26253).

Immunofluorescence:

ImmunofluorescenceofcellsexpressingFGFR1proteinswithanti-FGFR1(P252T)antibody.HEK293TcellsweretransfectedwithpCDNA3-GFP-FGFR1(WT)plasmid(leftcolumn)orpCDNA3-GFP-FGFR1(P252T)plasmid(rightcolumn),thenfixedandstainedwithanti-FGFR1(P252T)monoclonalantibody(Cat.#26253).

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