CatalogNumber:  21200

GeneSymbol:  ERBB2/HER2/Neu/c-Neu

Description:  Anti-ERBB2RabbitpolyclonalAntibody

Background:  TheHER-2/neuoncogene,amemberoftheepidermalgrowthfactorreceptororerb-Bgene-likefamily,encodesatransmembranetyrosinekinasereceptorthatmediatesextracellularsignalsactivatedbyepidermalgrowthfactors.Her2abnormalhasbeenstronglyassociatedwithmanymalignanttumors,especiallywithbreastcancers.TheexpressionlevelofHer2isanimportantcritiriainclinicevaluatingoftheprogressionofbreastcancer.

Immunogen:  AsyntheticpeptidefromtheC-terminalregionofERBB2,humanorigin.

Applications:  ELISA,WB,IHC

RecommendedDilutions:  ELISA:1:1000-1:5000WB:1:100-1:1000IHC:1:50-1:100

Concentration:  0.1mg/ml

Host:  Rabbit

Format:  Liquid

Clonality:  Polyclonal

Isotype:

Purity:  Purifiedfromserum

Storagebuffer:

Preservative:  no

Constituents:  PBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,50%glycerol

SpeciesReactivity:  RecognizesErb-B2ofvertebrates.

StorageConditions:  Storeat-20°C.Avoidfreeze/thawcycles.

DownloadDatasheet:

Immunohistochemistry:

Immunohistochemicalanalysisofparaffin-embeddedhumanlivercancertissuewithanti-Erb-B2polyclonalantibody(Cat.#21200).Tissuesampleswerefixedwithformaldehydeandblockedwith1%serumfor15minat37°C.Antigenretrievalwasbyheatmediationincitratebuffer(pH6).Sampleswerethenincubatedwithprimaryantibody(1:50)overnightat4°C.AHRP-conjugatedGoatanti-mouseIgG(dilution1:50)wasusedassecondaryantibody.

RelatedProducts
Catalog#	Name	Size	Price	DataSheet
21200	Anti-ERBB2RabbitpolyclonalAntibody	100μL	$219
21202	Anti-ERBB2RabbitPolyclonalAntibody	100μL	$219
26080	Anti-ERBB2(V777A)mousemonoclonalAntibody	100μL	$495
26127	Anti-ERBB2mousemonoclonalAntibody	100μL	$239

定制肽合成NewEast Biosciences为全球生物研究界提供高质量的粗制和纯化肽或拟肽。我们使用先进的自动，半自动和手动合成器以及Fmoc技术合成肽。肽酰胺键通过HOAT / HATU偶联形成。我们的肽通过制备型HPLC纯化，并且每个级分均通过使用不同溶剂系统的分析型HPLC进行进一步分析。收集并冻干仅通过质谱法验证的具有单个峰的级分。肽通常在1-2周内递送。粗肽也可以大量节省（参见价格表）。周转时间为5-7天。除了正常的肽合成外，我们还合成修饰的肽和多种抗原性肽（MAPs）。修饰在C末端，N末端和内部残基处进行。在C末端，氨基可与羧酸，异氰酸酯和磺酰氯反应形成酰胺，脲和磺酰胺。在此终端进行经典的生物素化，荧光标记和脂质化。这些修饰以及磷酸化和糖基化也可以在内部残基处进行。相反，在C末端的修饰非常具有挑战性，需要溶液阶段的干预。为了在C端进行修饰，必须将肽从树脂中释放出来。然后，用相应的化学方法修饰释放的肽。但是，我们已经开发了适当的化学方法，可以在固相的C末端引入生物素，荧光染料和胺。随着化学，我们降低了合成肽以及昂贵试剂的成本。作为NewEast的客户，我们将节省下来的钱转嫁给您。